In vivo semen characterization and seasonal variation in Procavia capensis

Seasonal reproduction is common among wild mammals, but male fertility traits are often understudied. The rock hyrax (Procavia capensis) is a seasonal breeder with a narrow reproductive window, yet its semen characteristics and seasonal variation remain poorly understood. Our objectives were to develop and validate anelectroejaculation protocol for semen collection, and to enable, for the first time, in vivo assessments of sperm morphology, ultrastructure, morphometry, and seasonal variation in semen quality in both captive and wild populations. A total of 70 semen collection attempts wereconducted: 17 in captive males at approximately monthly intervals over 1 year and 53 in wild males just before peak matingseasonandagain2–4weekslater,across3consecutiveyears(2021–2023).Electroejaculation was well to lerated and effective, particularly around the mating season, eliciting consistent responses and yielding sperm-containing ejaculates in 88.7% of wild procedures. Sperm morphometry revealed a mean total length of ∼56 µm, with ultrastructural features resembling other eutherian mammals. Seasonal analysis demonstrated significantly higher sperm concentration, motility, and normal morphology during peak mating season compared to later samples. Post-peak samples showed increased structural abnormalities, including midpiece and principal piece defects, and signs of disruptedspermatogenesisandepididymalmaturation.Cytologyandhigh-resolutionscanningelectronmicroscopyconfirmedthese findings, revealing compromised sperm integrity and elevated round cell counts outside the breeding peak. This study establishes the first in vivo semen collection protocol and comprehensive semen evaluation in the rock hyrax, revealing seasonal variation in male fertility and enabling repeatable, non-lethal reproductive monitoring.