In vivo expression of inducible nitric oxide synthase in cerebellar neurons

Dahlia Minc-Golomb, Gal Yadid, Ilan Tsarfaty, James H. Resau, Joan P. Schwartz

Research output: Contribution to journalArticlepeer-review

78 Scopus citations

Abstract

In the CNS, nitric oxide (NO) functions as both neuromodulator and neurotoxic agent. In vivo neuronal expression of NO synthase (NOS) has been attributed to constitutive NOS-both the neuronal and the endothelial types. The other class of NOS-the inducible NOS (iNOS)-is known to mediate toxic effects of NO in various tissues. In this study, we show for the first time that direct intracerebellar injection of endotoxin and cytokine (lipopolysaccharide and interferon-γ) induced in vivo neuronal expression of the iNOS gene, as demonstrated by fluorescent in situ hybridization and immunohistochemical staining analyzed by confocal laser-scanning microscopy. This raises the possibility that neuronal iNOS might contribute significantly to the vulnerability of the brain to various insults.

Original languageEnglish
Pages (from-to)1504-1509
Number of pages6
JournalJournal of Neurochemistry
Volume66
Issue number4
DOIs
StatePublished - Apr 1996

Keywords

  • Cerebellum
  • Cytokine
  • Endotoxin
  • Immunohistochemistry
  • In situ hybridization
  • Neuronal induction
  • Nitric oxide
  • Nitric oxide synthase

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