In vivo effects of porphyrins on bacterial DNA

Uri Nir, Hava Ladan, Zvi Malik, Yeshayahu Nitzan

    Research output: Contribution to journalArticlepeer-review

    39 Scopus citations

    Abstract

    The DNA damage in intact Staphylococcus aures and E. coli cells induced by photosensitized deuteroporphyrin or hemin is described. Treatment of S. aureus cultures with hemin or photosensitized deuteroporphyrin (Dp) caused time-dependent changes in the plasmidial DNA profiles. The major observation was the disappearance of the plasmid supercoiled fraction. The chromosomal DNA was also affected by hemin and by photosensitized Dp, since its degradation products were detected after exposing the bacterial cells to the porphyrin drugs. Photosensitization of E. coli cells, pretreated with Dp and polymyxin B nonapeptide (PMBNP), also resulted in plasmidial damage. No such damage occurred when E. coli cultures were treated with hemin and PMBNP. The above results can be tightly correlated with the antimicrobial action of porphyrins. Their damage to the bacterial DNA seems to reflect one of the in vivonagbibilang pa nga eh effects of these porphyrins.

    Original languageEnglish
    Pages (from-to)295-306
    Number of pages12
    JournalJournal of Photochemistry and Photobiology B: Biology
    Volume11
    Issue number3-4
    DOIs
    StatePublished - Dec 1991

    Keywords

    • E. coli
    • Gram negative
    • Gram positive
    • Porphyrins
    • Staphylococcus
    • antimicrobial agents
    • chromosome
    • plasmid DNA
    • polymyxin B nona-peptide.

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