In vitro studies on cell structure and function of human endothelial and osteoblast cocultures on fibroin nets

R. E. Unger, K. Peters, A. Motta, C. Migliaresi, C. J. Kirkpatrick

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

The cell compatibility and functions of human endothelial and osteoblast cocultures on fibroin nets were investigated. Primary human endothelial cells, umbilical cord (HUVEC), skin, the human endothelial cell lines HPMEC-ST1.6R, ISO-HAS-c1 were isolated and cultured. Cell growth on the material was visualized by confocal microscopy after staining of cells with Calcein-AM or by scanning electron microscopy (SEM). It was found that the coculture model system allows analysis of the interaction of cocultured cells visualized in a 3-D manner without disrupting cell culture or integrity.

Original languageEnglish
Title of host publicationTransactions - 7th World Biomaterials Congress
Pages659
Number of pages1
StatePublished - 2004
Externally publishedYes
EventTransactions - 7th World Biomaterials Congress - Sydney, Australia
Duration: 17 May 200421 May 2004

Publication series

NameTransactions - 7th World Biomaterials Congress

Conference

ConferenceTransactions - 7th World Biomaterials Congress
Country/TerritoryAustralia
CitySydney
Period17/05/0421/05/04

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