In Vitro Model for Studying of the Role of IGFBP6 Gene in Breast Cancer Metastasizing

S. V. Nikulin, M. P. Raigorodskaya, A. A. Poloznikov, G. S. Zakharova, U. Schumacher, D. Wicklein, C. Stürken, K. Riecken, K. A. Fomicheva, B. Ya Alekseev, M. Yu Shkurnikov

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


IGFBP6 gene plays an important role in the pathogenesis of breast cancer. In this work, we performed knockdown of IGFBP6 gene in MDA-MB-231 cells and obtained a stable cell line. Knockdown of IGFBP6 gene was confirmed by the real-time PCR. The influence of IGFBP6 gene on migration and proliferation of breast cancer cells was studied. Knockdown of IGFBP6 gene reduced migration activity of MDA-MB-231 cells and increased their proliferation rate. This in vitro cell model can be used for the further analysis of the role of IGFBP6 gene in the pathogenesis of breast cancer.

Original languageEnglish
Pages (from-to)688-692
Number of pages5
JournalBulletin of Experimental Biology and Medicine
Issue number5
StatePublished - 1 Mar 2018
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2018, Springer Science+Business Media, LLC, part of Springer Nature.


The study was supported by the Federal Target Program “Research and Development in Priority Areas of Advancement of the Russian Scientific and Technological Complex for 2014-2020” (RFME-FI61316X0058).

FundersFunder number
Federal Target ProgramRFME-FI61316X0058


    • IGFBP6
    • MDA-MB-231
    • breast cancer
    • in vitro model
    • metastasis spreading


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