In vitro and in vivo evaluation of carboplatin delivery to the eye using hydrogel-iontophoresis

Esther Eljarrat-Binstock, Abraham J. Domb, Faik Orucov, Amir Dagan, Joseph Frucht-Pery, Jacob Pe'Er

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

Purpose: To investigate in vitro and in vivo hydrogel-iontophoresis delivery of carboplatin to the eye. Methods: Iontophoresis was applied on agar gels resembling the eye using different current intensities and durations. Transscleral iontophoresis was performed on healthy rabbits, applying 0, 1, and 3 mA current for 10 min. Results: Similar drug concentrations were obtained in all experimental groups, in in vitro and in vivo studies, regardless of the iontophoretic current applied. A 20-mm penetration depth was found for carboplatin at the agar model. High drug levels were found at the sclera and retina, while lower levels were found at ocular fluids. Conclusion: Carboplatin-iontophoretic application at the above conditions does not have an obvious advantage over passive penetration due to high diffusion properties and insufficient molecular charge. Passive carboplatin diffusion from loaded hydrogels inserted in the lower cul-de-sac should be further investigated as a potential clinical treatment for intraocular retinoblastoma.

Original languageEnglish
Pages (from-to)269-275
Number of pages7
JournalCurrent Eye Research
Volume33
Issue number3
DOIs
StatePublished - Mar 2008
Externally publishedYes

Keywords

  • Carboplatin
  • Eye
  • Hydrogel
  • Iontophoresis
  • Retinoblastoma

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