In vitro and computational studies of transdermal perfusion of nanoformulations containing a large molecular weight protein

Madalena Martins; Nuno G. Azoia; Artur Ribeiro; Ulyana Shimanovich; Carla Silva; Artur Cavaco-Paulo

doi:10.1016/j.colsurfb.2013.02.032

In vitro and computational studies of transdermal perfusion of nanoformulations containing a large molecular weight protein

Madalena Martins, Nuno G. Azoia, Artur Ribeiro, Ulyana Shimanovich, Carla Silva, Artur Cavaco-Paulo

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

Transdermal perfusion of a large protein is reported for the first time, using a nanoemulsion of bovine serum albumin (66. kDa) of 160. nm prepared by a solid-in-oil (S/O) process. Molecular dynamics simulations confirmed skin permeation by these formulations, with integration of the protein into the lipid bilayers. These results demonstrate the real possibility of delivering large proteins transdermally for a range of medical and cosmetic applications.

Original language	English
Pages (from-to)	271-278
Number of pages	8
Journal	Colloids and Surfaces B: Biointerfaces
Volume	108
DOIs	https://doi.org/10.1016/j.colsurfb.2013.02.032
State	Published - 1 Aug 2013
Externally published	Yes

Bibliographical note

Funding Information:
We thank Matadouro - Central Carnes de Entre Douro e Minho, Lda for their support on pig samples. The histological studies were supported by the Department of Histology from Life and Health Sciences Research Institute (ICVS), University of Minho. The confocal 3D images were performed by Department of Chemistry and Kanbar Laboratory for Nanomaterials Bar-Ilan University Center for Advanced Materials and Nanotechnology. Carla Silva would like to thanks Fundação para a Ciência e a Tecnologia for their funding ( SFRH/BPD/46515/2008 ).

Funding

We thank Matadouro - Central Carnes de Entre Douro e Minho, Lda for their support on pig samples. The histological studies were supported by the Department of Histology from Life and Health Sciences Research Institute (ICVS), University of Minho. The confocal 3D images were performed by Department of Chemistry and Kanbar Laboratory for Nanomaterials Bar-Ilan University Center for Advanced Materials and Nanotechnology. Carla Silva would like to thanks Fundação para a Ciência e a Tecnologia for their funding ( SFRH/BPD/46515/2008 ).

Funders	Funder number
ICVS
Life and Health Sciences Research Institute
Fundação para a Ciência e a Tecnologia	SFRH/BPD/46515/2008
Universidade do Minho

Keywords

Hydrophilic macromolecules
Nanodispersion
Protein
Surfactant
Transdermal perfusion

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10.1016/j.colsurfb.2013.02.032

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abstract = "Transdermal perfusion of a large protein is reported for the first time, using a nanoemulsion of bovine serum albumin (66. kDa) of 160. nm prepared by a solid-in-oil (S/O) process. Molecular dynamics simulations confirmed skin permeation by these formulations, with integration of the protein into the lipid bilayers. These results demonstrate the real possibility of delivering large proteins transdermally for a range of medical and cosmetic applications.",

keywords = "Hydrophilic macromolecules, Nanodispersion, Protein, Surfactant, Transdermal perfusion",

author = "Madalena Martins and Azoia, {Nuno G.} and Artur Ribeiro and Ulyana Shimanovich and Carla Silva and Artur Cavaco-Paulo",

note = "Funding Information: We thank Matadouro - Central Carnes de Entre Douro e Minho, Lda for their support on pig samples. The histological studies were supported by the Department of Histology from Life and Health Sciences Research Institute (ICVS), University of Minho. The confocal 3D images were performed by Department of Chemistry and Kanbar Laboratory for Nanomaterials Bar-Ilan University Center for Advanced Materials and Nanotechnology. Carla Silva would like to thanks Funda{\c c}{\~a}o para a Ci{\^e}ncia e a Tecnologia for their funding ( SFRH/BPD/46515/2008 ).",

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AU - Ribeiro, Artur

AU - Shimanovich, Ulyana

AU - Silva, Carla

AU - Cavaco-Paulo, Artur

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In vitro and computational studies of transdermal perfusion of nanoformulations containing a large molecular weight protein

Abstract

Bibliographical note

Funding

Keywords

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