In situ super-resolution imaging of genomic DNA with oligoSTORM and oligoDNA-PAINT

Brian J. Beliveau, Alistair N. Boettiger, Guy Nir, Bogdan Bintu, Peng Yin, Xiaowei Zhuang, C. ting Wu

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

62 Scopus citations

Abstract

OligoSTORM and OligoDNA-PAINT meld the Oligopaint technology for fluorescent in situ hybridization (FISH) with, respectively, Stochastic Optical Reconstruction Microscopy (STORM) and DNA-based Point Accumulation for Imaging in Nanoscale Topography (DNA-PAINT) to enable in situ single-molecule super-resolution imaging of nucleic acids. Both strategies enable ≤20 nm resolution and are appropriate for imaging nanoscale features of the genomes of a wide range of species, including human, mouse, and fruit fly (Drosophila).

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages231-252
Number of pages22
DOIs
StatePublished - 2017
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1663
ISSN (Print)1064-3745

Bibliographical note

Publisher Copyright:
© 2017, Springer Science+Business Media LLC.

Funding

FundersFunder number
National Institute of General Medical SciencesR01GM105637

    Keywords

    • Chromatin
    • Chromosomes
    • DNA-PAINT
    • FISH
    • Genome
    • OligoDNA-PAINT
    • OligoSTORM
    • Oligopaint
    • STORM
    • Single-molecule
    • Super-resolution

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