In-Situ Architectures Designed in 3D Cell-Laden Hydrogels Using Microscopic Laser Photolithography

Iris Mironi-Harpaz; Lena Hazanov; Guy Engel; Dvir Yelin; Dror Seliktar

doi:10.1002/adma.201404185

In-Situ Architectures Designed in 3D Cell-Laden Hydrogels Using Microscopic Laser Photolithography

Iris Mironi-Harpaz, Lena Hazanov, Guy Engel, Dvir Yelin, Dror Seliktar

Technion-Israel Institute of Technology

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

Thermoresponsive bioactive hydrogels made from fibrinogen and Pluronic F127 are physically crosslinked at 37 °C to facilitate local chemical crosslinking using microscopic photolithography. The reaction conditions are mild enough to pattern in the presence of living cells. The interfaces between the chemically crosslinked "stiffer" and physically crosslinked "softer" regions capture the unique displays of morphogenesis in response to changing stiffness at the cellular scale.

Original language	English
Pages (from-to)	1933-1938
Number of pages	6
Journal	Advanced Materials
Volume	27
Issue number	11
DOIs	https://doi.org/10.1002/adma.201404185
State	Published - 18 Mar 2015
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2015 Wiley-VCH Verlag GmbH & Co. KGaA.

Funding

Funders	Funder number
European Commission
Seventh Framework Programme	239986

Keywords

3D cell culture
free-radical polymerization
microrheology
microscopic photopatterning
reverse thermal gelation

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10.1002/adma.201404185

Cite this

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AU - Yelin, Dvir

AU - Seliktar, Dror

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AB - Thermoresponsive bioactive hydrogels made from fibrinogen and Pluronic F127 are physically crosslinked at 37 °C to facilitate local chemical crosslinking using microscopic photolithography. The reaction conditions are mild enough to pattern in the presence of living cells. The interfaces between the chemically crosslinked "stiffer" and physically crosslinked "softer" regions capture the unique displays of morphogenesis in response to changing stiffness at the cellular scale.

KW - 3D cell culture

KW - free-radical polymerization

KW - microrheology

KW - microscopic photopatterning

KW - reverse thermal gelation

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In-Situ Architectures Designed in 3D Cell-Laden Hydrogels Using Microscopic Laser Photolithography

Abstract

Bibliographical note

Funding

Keywords

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