Improving the sensitivity of fluorescence-based immunoassays by photobleaching the autofluorescence of magnetic beads

Shira Roth; Orr Hadass; Meir Cohen; Jasenka Verbarg; Jennifer Wilsey; Amos Danielli

doi:10.1117/12.2507597

Improving the sensitivity of fluorescence-based immunoassays by photobleaching the autofluorescence of magnetic beads

Shira Roth, Orr Hadass, Meir Cohen, Jasenka Verbarg, Jennifer Wilsey, Amos Danielli

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

One of the most common capture surfaces is a magnetic bead. However, magnetic beads exhibit strong autofluorescence, which often overlaps with the emission of the reporter fluorescent dyes and limits the analytical sensitivity of the assay. Here, we photobleached several widely used magnetic beads and reduced their autofluorescence to 1% of the initial value. The photobleached beads were stable over time and their surface functionality was retained. In a high sensitivity LX-200™ system using photobleached magnetic beads, human interleukin-8 was detected with a 3-fold improvement in analytical sensitivity and signal to noise ratio over results achievable with non-bleached beads.

Original language	English
Title of host publication	Frontiers in Biological Detection
Subtitle of host publication	From Nanosensors to Systems XI
Editors	Amos Danielli, Benjamin L. Miller, Sharon M. Weiss
Publisher	SPIE
ISBN (Electronic)	9781510624320
DOIs	https://doi.org/10.1117/12.2507597
State	Published - 2019
Event	Frontiers in Biological Detection: From Nanosensors to Systems XI 2019 - San Francisco, United States Duration: 3 Feb 2019 → 4 Feb 2019

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	10895
ISSN (Print)	1605-7422

Conference

Conference	Frontiers in Biological Detection: From Nanosensors to Systems XI 2019
Country/Territory	United States
City	San Francisco
Period	3/02/19 → 4/02/19

Bibliographical note

Funding Information:
This research was partially supported by the Israel Science Foundation (grant No. 1142/15) and the Israel Ministry of Science and Technology (National foundation for practical and engineering science). A.D. has a financial interest in MagBiosense, Inc. This paper is a small part of a work that was published in "Small" journal. For the full research results please refer to the full text, DOI: 10.1002/smll.201803751.

Publisher Copyright:
© 2019 SPIE.

Keywords

Autofluorescence
Fluorescence-based immunoassays
Magnetic beads
Magnetic modulation biosensing
Photobleaching

Access to Document

10.1117/12.2507597

Cite this

Roth, S., Hadass, O., Cohen, M., Verbarg, J., Wilsey, J., & Danielli, A. (2019). Improving the sensitivity of fluorescence-based immunoassays by photobleaching the autofluorescence of magnetic beads. In A. Danielli, B. L. Miller, & S. M. Weiss (Eds.), Frontiers in Biological Detection: From Nanosensors to Systems XI [1089509] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10895). SPIE. https://doi.org/10.1117/12.2507597

Roth, Shira ; Hadass, Orr ; Cohen, Meir et al. / Improving the sensitivity of fluorescence-based immunoassays by photobleaching the autofluorescence of magnetic beads. Frontiers in Biological Detection: From Nanosensors to Systems XI. editor / Amos Danielli ; Benjamin L. Miller ; Sharon M. Weiss. SPIE, 2019. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE).

@inproceedings{8814b27f08f34e78ac44b125934c801a,

title = "Improving the sensitivity of fluorescence-based immunoassays by photobleaching the autofluorescence of magnetic beads",

abstract = "One of the most common capture surfaces is a magnetic bead. However, magnetic beads exhibit strong autofluorescence, which often overlaps with the emission of the reporter fluorescent dyes and limits the analytical sensitivity of the assay. Here, we photobleached several widely used magnetic beads and reduced their autofluorescence to 1% of the initial value. The photobleached beads were stable over time and their surface functionality was retained. In a high sensitivity LX-200{\texttrademark} system using photobleached magnetic beads, human interleukin-8 was detected with a 3-fold improvement in analytical sensitivity and signal to noise ratio over results achievable with non-bleached beads.",

keywords = "Autofluorescence, Fluorescence-based immunoassays, Magnetic beads, Magnetic modulation biosensing, Photobleaching",

author = "Shira Roth and Orr Hadass and Meir Cohen and Jasenka Verbarg and Jennifer Wilsey and Amos Danielli",

note = "Publisher Copyright: {\textcopyright} 2019 SPIE.; Frontiers in Biological Detection: From Nanosensors to Systems XI 2019 ; Conference date: 03-02-2019 Through 04-02-2019",

year = "2019",

doi = "10.1117/12.2507597",

language = "אנגלית",

series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

publisher = "SPIE",

editor = "Amos Danielli and Miller, {Benjamin L.} and Weiss, {Sharon M.}",

booktitle = "Frontiers in Biological Detection",

address = "ארצות הברית",

}

Roth, S, Hadass, O, Cohen, M, Verbarg, J, Wilsey, J & Danielli, A 2019, Improving the sensitivity of fluorescence-based immunoassays by photobleaching the autofluorescence of magnetic beads. in A Danielli, BL Miller & SM Weiss (eds), Frontiers in Biological Detection: From Nanosensors to Systems XI., 1089509, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 10895, SPIE, Frontiers in Biological Detection: From Nanosensors to Systems XI 2019, San Francisco, United States, 3/02/19. https://doi.org/10.1117/12.2507597

Improving the sensitivity of fluorescence-based immunoassays by photobleaching the autofluorescence of magnetic beads. / Roth, Shira; Hadass, Orr; Cohen, Meir et al.
Frontiers in Biological Detection: From Nanosensors to Systems XI. ed. / Amos Danielli; Benjamin L. Miller; Sharon M. Weiss. SPIE, 2019. 1089509 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10895).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Improving the sensitivity of fluorescence-based immunoassays by photobleaching the autofluorescence of magnetic beads

AU - Roth, Shira

AU - Hadass, Orr

AU - Cohen, Meir

AU - Verbarg, Jasenka

AU - Wilsey, Jennifer

AU - Danielli, Amos

PY - 2019

Y1 - 2019

N2 - One of the most common capture surfaces is a magnetic bead. However, magnetic beads exhibit strong autofluorescence, which often overlaps with the emission of the reporter fluorescent dyes and limits the analytical sensitivity of the assay. Here, we photobleached several widely used magnetic beads and reduced their autofluorescence to 1% of the initial value. The photobleached beads were stable over time and their surface functionality was retained. In a high sensitivity LX-200™ system using photobleached magnetic beads, human interleukin-8 was detected with a 3-fold improvement in analytical sensitivity and signal to noise ratio over results achievable with non-bleached beads.

AB - One of the most common capture surfaces is a magnetic bead. However, magnetic beads exhibit strong autofluorescence, which often overlaps with the emission of the reporter fluorescent dyes and limits the analytical sensitivity of the assay. Here, we photobleached several widely used magnetic beads and reduced their autofluorescence to 1% of the initial value. The photobleached beads were stable over time and their surface functionality was retained. In a high sensitivity LX-200™ system using photobleached magnetic beads, human interleukin-8 was detected with a 3-fold improvement in analytical sensitivity and signal to noise ratio over results achievable with non-bleached beads.

KW - Autofluorescence

KW - Fluorescence-based immunoassays

KW - Magnetic beads

KW - Magnetic modulation biosensing

KW - Photobleaching

UR - http://www.scopus.com/inward/record.url?scp=85068060606&partnerID=8YFLogxK

U2 - 10.1117/12.2507597

DO - 10.1117/12.2507597

M3 - ???researchoutput.researchoutputtypes.contributiontobookanthology.conference???

AN - SCOPUS:85068060606

T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

BT - Frontiers in Biological Detection

A2 - Danielli, Amos

A2 - Miller, Benjamin L.

A2 - Weiss, Sharon M.

PB - SPIE

T2 - Frontiers in Biological Detection: From Nanosensors to Systems XI 2019

Y2 - 3 February 2019 through 4 February 2019

ER -

Roth S, Hadass O, Cohen M, Verbarg J, Wilsey J, Danielli A. Improving the sensitivity of fluorescence-based immunoassays by photobleaching the autofluorescence of magnetic beads. In Danielli A, Miller BL, Weiss SM, editors, Frontiers in Biological Detection: From Nanosensors to Systems XI. SPIE. 2019. 1089509. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). doi: 10.1117/12.2507597

Improving the sensitivity of fluorescence-based immunoassays by photobleaching the autofluorescence of magnetic beads

Abstract

Publication series

Conference

Bibliographical note

Keywords

Access to Document

Other files and links

Fingerprint

Cite this