One of the most common capture surfaces is a magnetic bead. However, magnetic beads exhibit strong autofluorescence, which often overlaps with the emission of the reporter fluorescent dyes and limits the analytical sensitivity of the assay. Here, we photobleached several widely used magnetic beads and reduced their autofluorescence to 1% of the initial value. The photobleached beads were stable over time and their surface functionality was retained. In a high sensitivity LX-200™ system using photobleached magnetic beads, human interleukin-8 was detected with a 3-fold improvement in analytical sensitivity and signal to noise ratio over results achievable with non-bleached beads.
|Title of host publication||Frontiers in Biological Detection|
|Subtitle of host publication||From Nanosensors to Systems XI|
|Editors||Amos Danielli, Benjamin L. Miller, Sharon M. Weiss|
|State||Published - 2019|
|Event||Frontiers in Biological Detection: From Nanosensors to Systems XI 2019 - San Francisco, United States|
Duration: 3 Feb 2019 → 4 Feb 2019
|Name||Progress in Biomedical Optics and Imaging - Proceedings of SPIE|
|Conference||Frontiers in Biological Detection: From Nanosensors to Systems XI 2019|
|Period||3/02/19 → 4/02/19|
Bibliographical noteFunding Information:
This research was partially supported by the Israel Science Foundation (grant No. 1142/15) and the Israel Ministry of Science and Technology (National foundation for practical and engineering science). A.D. has a financial interest in MagBiosense, Inc. This paper is a small part of a work that was published in "Small" journal. For the full research results please refer to the full text, DOI: 10.1002/smll.201803751.
© 2019 SPIE.
- Fluorescence-based immunoassays
- Magnetic beads
- Magnetic modulation biosensing