Implanted neonatal human dermal fibroblasts influence the recruitment of endothelial cells in mice.

Susana G. Guerreiro, Christoph Brochhausen, Rita Negrão, Mário A. Barbosa, Ronald E. Unger, C. James Kirkpatrick, Raquel Soares, Pedro L. Granja

Research output: Contribution to journalArticlepeer-review

14 Scopus citations


The vascularization of new tissue within a reasonable time is a crucial prerequisite for the success of different cell- and material-based strategies. Considering that angiogenesis is a multi-step process involving humoral and cellular regulatory components, only in vivo assays provide the adequate information about vessel formation and the recruitment of endothelial cells. The present study aimed to investigate if neonatal human dermal fibroblasts could influence in vivo neovascularization. Results obtained showed that fibroblasts were able to recruit endothelial cells to vascularize the implanted matrix, which was further colonized by murine functional blood vessels after one week. The vessels exhibited higher levels of hemoglobin, compared with the control matrix, implanted without fibroblasts, in which no vessel formation could be observed. No significant differences were detected in systemic inflammation. The presence of vessels originated from the host vasculature suggested that host vascular response was involved, which constitutes a fundamental aspect in the process of neovascularization. Fibroblasts implanted within matrigel increased the presence of endothelial cells with positive staining for CD31 and for CD34 and the production of collagen influencing the angiogenic process and promoting the formation of microvessels. New strategies in tissue engineering could be delineated with improved angiogenesis using neonatal fibroblasts.

Original languageEnglish
Pages (from-to)43-52
Number of pages10
Issue number1
StatePublished - 2012
Externally publishedYes

Bibliographical note

Funding Information:
The work performed at INEB was financed by FEDER funds through the Programa Operacional Factores de Competitividade— COMPETE and by Portuguese funds through FCT—Fundação para a Ciência e a Tecnologia in the framework of projects PEST-C/SAU/LA0002/2011 and POCI/SAU-BMA/55556/2004 and the PhD scholarship SFRH/BD/40421/2007 for S.G.G.


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