TY - JOUR
T1 - Immunoaffinity purification
T2 - Basic principles and operational considerations
AU - Yarmush, M. L.
AU - Weiss, A. M.
AU - Antonsen, K. P.
AU - Odde, D. J.
AU - Yarmush, D. M.
PY - 1992
Y1 - 1992
N2 - Immunoaffinity purification has become an important technique in biotechnology. In this review the basic principles of immunoaffinity separations are described with respect to the stages of operation and potential application. The most commonly used support materials, activation procedures, and coupling chemistries are compared to one another for suitability in various applications. Individual operational steps for fixed bed immunoadsorbents including loading, washing, elution and regeneration are described in terms of both theory and practice. Factors influencing adsorbent stability are identified, and alternative operation and configuration strategies are discussed in light of their application to immunoaffinity systems.
AB - Immunoaffinity purification has become an important technique in biotechnology. In this review the basic principles of immunoaffinity separations are described with respect to the stages of operation and potential application. The most commonly used support materials, activation procedures, and coupling chemistries are compared to one another for suitability in various applications. Individual operational steps for fixed bed immunoadsorbents including loading, washing, elution and regeneration are described in terms of both theory and practice. Factors influencing adsorbent stability are identified, and alternative operation and configuration strategies are discussed in light of their application to immunoaffinity systems.
KW - Immunoaffinity purification
KW - affinity adsorption
KW - affinity chromatography
KW - immobilization
KW - immunoaffinity chromatography
KW - monoclonal antibodies
UR - http://www.scopus.com/inward/record.url?scp=0001524095&partnerID=8YFLogxK
U2 - 10.1016/0734-9750(92)90303-q
DO - 10.1016/0734-9750(92)90303-q
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AN - SCOPUS:0001524095
SN - 0734-9750
VL - 10
SP - 413
EP - 446
JO - Biotechnology Advances
JF - Biotechnology Advances
IS - 3
ER -