Immobilized polymeric microspheres: Polyacrolein microspheres covalently bound in a monolayer structure onto glass surfaces

Shlomo Margel, Yehudit Dolitzky, Orit Sivan

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Novel materials composed of an unpacked monolayer of polyacrolein microspheres covalently bonded onto glass surfaces have been prepared and characterized. These surfaces were synthesized by the covalent binding of long- and short-chain length ω-cyano-alkylsilane compounds (SiCl3(CH2)nCN and Si(OMe)3(CH2)nCN, where n = 3 and 16) onto glass substrates. The nitrile groups at the ω position were then reduced to primary amino groups. Polyacrolein microspheres of 0.7 μm average diameter were then bonded to the modified surfaces. This binding of the microspheres to the derivatized surfaces is achieved via polyvalent Schiff-base bonds formed by the interaction between aldehyde groups of the microspheres and ω-primary amino groups of the derivatized glass surfaces. The percent coverage of the glass surfaces by the microspheres was higher for surfaces coated with the trichloroalkylsilane compounds compared to trimethoxyalkylsilane compounds, and higher also for surfaces coated with the short-chain length alkylsilane compounds compared to surfaces coated with the long ones. The residual aldehyde groups of the immobilized microspheres can then be used for the covalent binding of amino ligands, e.g. proteins, in a single step and at physiological pH (or any other desired pH). The binding kinetics of bungarotoxin to the immobilized microspheres has been investigated. Fourier transform-infrared/attenuated total reflection, electron spectroscopy for chemical analysis, contact angle measurements and scanning electron microscopy have been used for the characterization of the alkylsilane coatings and the binding of the microspheres to these coatings.

Original languageEnglish
Pages (from-to)215-230
Number of pages16
JournalColloids and Surfaces
Issue number3
StatePublished - 5 Feb 1992

Bibliographical note

Funding Information:
We thank A. Shainberg of Bar-Ilan University, Department of Life Sciences, Ramat-Gan, Israel for providing the “‘1-bungarotoxin and the facilities to carry out the experimentsw ith this radiolabeled protein. Thanks are also due t.o C. Sukenik and N. Balachander from Case Western Reserve University, Cleveland,O H for their help in carrying out, k::Id interpretation of, some of the ESCA studies. These studies have beenp artially supported by the Israeli National Council for Research and Development


  • Latexes
  • immobilized microspheres
  • microspheres
  • polyacrolein microspheres.


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