Immobilization of urease from pigeonpea (Cajanus cajan) on agar tablets and its application in urea assay

Swati Mulagalapalli, Sandeep Kumar, Ravi Charan Reddy Kalathur, Arvind M. Kayastha

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

The pigeonpea urease was immobilized on agar, a common gelling substance. The tablet strips were used as moulds to cast agar tablets of uniform shape and size. The time and temperature of solidification of agar was 6 min and 44°C, respectively. The 5 % agar (w/v) and 0.019 mg protein/agar tablet yielded an optimum immobilization of 51.7%. The optimum pH was shifted through 0.2 U (from 7.3 to 7.5) towards basic side upon immobilization. The optimum temperature of soluble and immobilized urease was 30°C and 60°C, respectively, showing the improvement in thermal stability of urease. There was an increase in K m from 3.23 to 5.07 mM after immobilization. The half-lives of soluble and immobilized urease were 21 and 53 days, respectively, at pH 7.3 and 4°C. The urea was estimated in different blood samples with the help of immobilized urease and the results were consistent with those from clinical pathology laboratory through an autoanalyzer® (Zydus Co., Rome, Italy).

Original languageEnglish
Pages (from-to)291-297
Number of pages7
JournalApplied Biochemistry and Biotechnology
Volume142
Issue number3
DOIs
StatePublished - Sep 2007
Externally publishedYes

Keywords

  • Agar
  • Cajanus cajan
  • Immobilization
  • Urea
  • Urease

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