TY - JOUR
T1 - Immobilization of urease from pigeonpea (Cajanus cajan) on agar tablets and its application in urea assay
AU - Mulagalapalli, Swati
AU - Kumar, Sandeep
AU - Kalathur, Ravi Charan Reddy
AU - Kayastha, Arvind M.
PY - 2007/9
Y1 - 2007/9
N2 - The pigeonpea urease was immobilized on agar, a common gelling substance. The tablet strips were used as moulds to cast agar tablets of uniform shape and size. The time and temperature of solidification of agar was 6 min and 44°C, respectively. The 5 % agar (w/v) and 0.019 mg protein/agar tablet yielded an optimum immobilization of 51.7%. The optimum pH was shifted through 0.2 U (from 7.3 to 7.5) towards basic side upon immobilization. The optimum temperature of soluble and immobilized urease was 30°C and 60°C, respectively, showing the improvement in thermal stability of urease. There was an increase in K m from 3.23 to 5.07 mM after immobilization. The half-lives of soluble and immobilized urease were 21 and 53 days, respectively, at pH 7.3 and 4°C. The urea was estimated in different blood samples with the help of immobilized urease and the results were consistent with those from clinical pathology laboratory through an autoanalyzer® (Zydus Co., Rome, Italy).
AB - The pigeonpea urease was immobilized on agar, a common gelling substance. The tablet strips were used as moulds to cast agar tablets of uniform shape and size. The time and temperature of solidification of agar was 6 min and 44°C, respectively. The 5 % agar (w/v) and 0.019 mg protein/agar tablet yielded an optimum immobilization of 51.7%. The optimum pH was shifted through 0.2 U (from 7.3 to 7.5) towards basic side upon immobilization. The optimum temperature of soluble and immobilized urease was 30°C and 60°C, respectively, showing the improvement in thermal stability of urease. There was an increase in K m from 3.23 to 5.07 mM after immobilization. The half-lives of soluble and immobilized urease were 21 and 53 days, respectively, at pH 7.3 and 4°C. The urea was estimated in different blood samples with the help of immobilized urease and the results were consistent with those from clinical pathology laboratory through an autoanalyzer® (Zydus Co., Rome, Italy).
KW - Agar
KW - Cajanus cajan
KW - Immobilization
KW - Urea
KW - Urease
UR - http://www.scopus.com/inward/record.url?scp=35048849832&partnerID=8YFLogxK
U2 - 10.1007/s12010-007-0022-7
DO - 10.1007/s12010-007-0022-7
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C2 - 18025589
AN - SCOPUS:35048849832
SN - 0273-2289
VL - 142
SP - 291
EP - 297
JO - Applied Biochemistry and Biotechnology
JF - Applied Biochemistry and Biotechnology
IS - 3
ER -