Imaging the effect of hemoglobin on properties of RBCs using common-path digital holographic microscope

M. Joglekar, H. Shah, V. Trivedi, S. Mahajan, V. Chhaniwal, R. Leitgeb, B. Javidi, A. Anand

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review


Adequate supply of oxygen to the body is the most essential requirement. In vertebrate species this function is performed by Hemoglobin contained in red blood cells. The mass concentration of the Hb determines the oxygen carrying capacity of the blood. Thus it becomes necessary to determine its concentration in the blood, which helps in monitoring the health of a person. If the amount of Hb crosses certain range, then it is considered critical. As the Hb constitutes upto 96% of red blood cells dry content, it would be interesting to examine various physical and mechanical parameters of RBCs which depends upon its concentration. Various diseases bring about significant variation in the amount of hemoglobin which may alter certain parameters of the RBC such as surface area, volume, membrane fluctuation etc. The study of the variations of these parameters may be helpful in determining Hb content which will reflect the state of health of a human body leading to disease diagnosis. Any increase or decrease in the amount of Hb will change the density and hence the optical thickness of the RBCs, which affects the cell membrane and thereby changing its mechanical and physical properties. Here we describe the use of lateral shearing digital holographic microscope for quantifying the cell parameters for studying the change in biophysical properties of cells due to variation in hemoglobin concentration.

Original languageEnglish
Title of host publicationAdvances in Microscopic Imaging
EditorsFrancesco Saverio Pavone, Emmanuel Beaurepaire, Peter T. C. So
ISBN (Electronic)9781510612860
StatePublished - 2017
Externally publishedYes
EventAdvances in Microscopic Imaging 2017 - Munich, Germany
Duration: 27 Jun 201729 Jun 2017

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


ConferenceAdvances in Microscopic Imaging 2017

Bibliographical note

Publisher Copyright:
© 2017 SPIE.


Arun Anand would like to acknowledge the financial assistance through research grant from DAE-BRNS (2013/34/11/BRNS/504). He also would like to thank UGC, Govt. of India and DST Govt. of India for departmental research grants through UGC-DRS and DST-FIST schemes. Vani K. Chhaniwal and Arun Anand would like to thank Abdus Salam ICTP, Trieste, Italy for the Regular Associate fellowship.

FundersFunder number


    • Bio-physical parameters
    • Cell 3D imaging
    • Digital holographic microscopy
    • Hemoglobin


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