Imaging gene expression in single living cells

Yaron Shav-Tal, Robert H. Singer, Xavier Darzacq

Research output: Contribution to journalReview articlepeer-review

88 Scopus citations

Abstract

Technical advances in the field of live-cell imaging have introduced the cell biologist to a new, dynamic, subcellular world. The static world of molecules in fixed cells has now been extended to the time dimension. This allows the visualization and quantification of gene expression and intracellular trafficking events of the studied molecules and the associated enzymatic processes in individual cells, in real time.

Original languageEnglish
Pages (from-to)855-861
Number of pages7
JournalNature Reviews Molecular Cell Biology
Volume5
Issue number10
DOIs
StatePublished - Oct 2004
Externally publishedYes

Bibliographical note

Funding Information:
We would like to thank B. Ovryn for critical reading of the manuscript. R.H.S is supported by the National Institutes of Health.

Funding Information:
I would like to apologize to those whose work was not cited due to space limitations. I would also like to thank R. Cross, C. Cvetic, J. Huberman, P. Kane, T. Melendy, C. Schildkraut, J. Walter and R. West for critical reading of the manuscript. D.M.G. is supported by the National Institutes of Health, the National Science Foundation and American Cancer Society.

Funding

We would like to thank B. Ovryn for critical reading of the manuscript. R.H.S is supported by the National Institutes of Health. I would like to apologize to those whose work was not cited due to space limitations. I would also like to thank R. Cross, C. Cvetic, J. Huberman, P. Kane, T. Melendy, C. Schildkraut, J. Walter and R. West for critical reading of the manuscript. D.M.G. is supported by the National Institutes of Health, the National Science Foundation and American Cancer Society.

FundersFunder number
National Science Foundation
National Institutes of Health
American Cancer Society
National Institute of Biomedical Imaging and BioengineeringR01EB002060

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