Identifying genes and regulatory pathways associated with the scleractinian coral calcification process

Eldad Gutner-Hoch, Hiba Waldman Ben-Asher, Ruth Yam, Aldo Shemesh, Oren Levy

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Reef building corals precipitate calcium carbonate as an exo-skeleton and provide substratum for prosperous marine life. Biomineralization of the coral's skeleton is a developmental process that occurs concurrently with other proliferation processes that control the animal extension and growth. The development of the animal body is regulated by large gene regulatory networks, which control the expression of gene sets that progressively generate developmental patterns in the animal body. In this study we have explored the gene expression profile and signaling pathways followed by the calcification process of a basal metazoan, the Red Sea scleractinian (stony) coral, Stylophora pistillata. When treated by seawater with high calcium concentrations (addition of 100 gm/L, added as CaCl2 .2H2 O), the coral increases its calcification rates and associated genes were up-regulated as a result, which were then identified. Gene expression was compared between corals treated with elevated and normal calcium concentrations. Calcification rate measurements and gene expression analysis by microarray RNA transcriptional profiling at two time-points (midday and night-time) revealed several genes common within mammalian gene regulatory networks. This study indicates that core genes of the Wnt and TGF-β/BMP signaling pathways may also play roles in development, growth, and biomineralization in early-diverging organisms such as corals.

Original languageEnglish
Article numbere3590
Pages (from-to)3590
Number of pages1
JournalPeerJ
Volume2017
Issue number7
DOIs
StatePublished - 2017

Bibliographical note

Publisher Copyright:
© 2017 Gutner-Hoch et al.

Keywords

  • Calcification
  • Gene expression
  • Microarray
  • Regulatory pathways
  • Scleractinian coral
  • TGF-beta/BMP pathway
  • Wnt pathway

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