Abstract
The construction and identification of a recombinant plasmid containing a cDNA Insert which hybridizes specifically to myosin heavy chain mRNA is described. The plasmid was used as a probe to screen a rat genomic library for recombinant phages containing myosin heavy chain sequences. Six clones with approximately 15 k bp inserts each were isolated. Digestion with several restriction enzymes and hybridization of the fractionated DNA with the plasmid probe showed that the clones contained 3 different DNA inserts. Electron microscopy of a heteroduplex made by hybridization of DNA from two clones confirmed that the inserts originated in different genes. Hybridization of size-fractionated EcoR1 digested rat spleen DNA with the cloned probe suggested the existence of at least 5 myosin heavy chain genes.
Original language | English |
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Pages (from-to) | 2133-2146 |
Number of pages | 14 |
Journal | Nucleic Acids Research |
Volume | 8 |
Issue number | 10 |
DOIs | |
State | Published - 24 May 1980 |
Externally published | Yes |
Bibliographical note
Funding Information:The work was supported by a grant from the Muscular Dystrophy Associa-and by Grant #R01-GM-22767 from the National Institutes of Health.
Funding
The work was supported by a grant from the Muscular Dystrophy Associa-and by Grant #R01-GM-22767 from the National Institutes of Health.
Funders | Funder number |
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National Institutes of Health | |
National Institute of General Medical Sciences | R01GM022767 |
French Muscular Dystrophy Association | 01-GM-22767 |