Identification of recombinant phages containing sequences from different rat myosin heavy chain genes

U. Nudel, D. Katcoff, Y. Carmon, D. Zevin-Sonkin, Z. Levi, Y. Shaul, M. Shani, D. Yaffe

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

The construction and identification of a recombinant plasmid containing a cDNA Insert which hybridizes specifically to myosin heavy chain mRNA is described. The plasmid was used as a probe to screen a rat genomic library for recombinant phages containing myosin heavy chain sequences. Six clones with approximately 15 k bp inserts each were isolated. Digestion with several restriction enzymes and hybridization of the fractionated DNA with the plasmid probe showed that the clones contained 3 different DNA inserts. Electron microscopy of a heteroduplex made by hybridization of DNA from two clones confirmed that the inserts originated in different genes. Hybridization of size-fractionated EcoR1 digested rat spleen DNA with the cloned probe suggested the existence of at least 5 myosin heavy chain genes.

Original languageEnglish
Pages (from-to)2133-2146
Number of pages14
JournalNucleic Acids Research
Volume8
Issue number10
DOIs
StatePublished - 24 May 1980
Externally publishedYes

Bibliographical note

Funding Information:
The work was supported by a grant from the Muscular Dystrophy Associa-and by Grant #R01-GM-22767 from the National Institutes of Health.

Funding

The work was supported by a grant from the Muscular Dystrophy Associa-and by Grant #R01-GM-22767 from the National Institutes of Health.

FundersFunder number
National Institutes of Health
National Institute of General Medical SciencesR01GM022767
French Muscular Dystrophy Association01-GM-22767

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