TY - JOUR
T1 - Identification of a tRNA-like molecule that copurifies with the 7SL RNA of Trypanosoma brucei
AU - Béjà, Oded
AU - Ullu, Elisabetta
AU - Michaeli, Shulamit
PY - 1993/2
Y1 - 1993/2
N2 - During the purification of Trypanosoma brucei 7SL RNA, we detected a small RNA, 76 nucleotide-long (sRNA-76), that copurified with the 7SL RNP through several different separation steps. In this study, a partial RNA sequence of sRNA-76 was obtained and a complementary oligonucleotide to the RNA sequence was used to clone the corresponding gene. sRNA-76 is very similar to a tRNA molecule and is encoded by a single copy gene. The gene is located next to a tRNAVal which has 75.3% homology to T. brucei tRNAVal that exists in a different chromosomal locus. The highest homology of sRNA-76 is to mouse and rat tRNAAsp (69%), to mouse tRNAGly (68.1%) and to yeast suppressor tRNAGly (69.5%). However, sRNA-76 is neither a tRNAAsp nor a tRNAGly, since it has a Leu anticodon. In addition, sRNA-76 deviates from the canonical tRNA structure in 3 positions. A potential for base pairing between sRNA-76 and 7SL RNA was found in the 100 nt region of 7SL RNA, which is a highly conserved region in all 7SL RNAs.
AB - During the purification of Trypanosoma brucei 7SL RNA, we detected a small RNA, 76 nucleotide-long (sRNA-76), that copurified with the 7SL RNP through several different separation steps. In this study, a partial RNA sequence of sRNA-76 was obtained and a complementary oligonucleotide to the RNA sequence was used to clone the corresponding gene. sRNA-76 is very similar to a tRNA molecule and is encoded by a single copy gene. The gene is located next to a tRNAVal which has 75.3% homology to T. brucei tRNAVal that exists in a different chromosomal locus. The highest homology of sRNA-76 is to mouse and rat tRNAAsp (69%), to mouse tRNAGly (68.1%) and to yeast suppressor tRNAGly (69.5%). However, sRNA-76 is neither a tRNAAsp nor a tRNAGly, since it has a Leu anticodon. In addition, sRNA-76 deviates from the canonical tRNA structure in 3 positions. A potential for base pairing between sRNA-76 and 7SL RNA was found in the 100 nt region of 7SL RNA, which is a highly conserved region in all 7SL RNAs.
KW - 7SL RNA
KW - Signal recognition particle
KW - Trypanosoma
KW - sRNA-76
KW - tRNA
UR - http://www.scopus.com/inward/record.url?scp=0027466121&partnerID=8YFLogxK
U2 - 10.1016/0166-6851(93)90198-7
DO - 10.1016/0166-6851(93)90198-7
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C2 - 8433714
AN - SCOPUS:0027466121
SN - 0166-6851
VL - 57
SP - 223
EP - 229
JO - Molecular and Biochemical Parasitology
JF - Molecular and Biochemical Parasitology
IS - 2
ER -