Identification and characterization of the regulated pattern of expression of a novel mouse gene, meg1, during the meiotic cell cycle.

J. Don, D. J. Wolgemuth

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25 Scopus citations

Abstract

The gene designated meg1 (meiosis expressed gene) is a new mouse gene identified during a search for mammalian genes potentially involved in meiotic processes. Two classes of complementary DNAs were isolated from an adult mouse testis complementary DNA library, which shared the same 3' end including the entire putative coding region but differed in their 5' ends. Only one of these complementary DNA classes appeared to correspond to the very abundant 0.75-kilobase testicular transcript of meg1. Sequence analysis predicts a 10.8-kilodalton protein which is highly charged and lysine rich. It is also relatively rich in potential phosphoacceptor amino acids (approximately 17%), several of which are located in phosphorylation consensus sequences. The pattern of expression of meg1 was studied utilizing a combined Northern blot and in situ hybridization analysis. Of the adult tissues examined, meg1 transcripts were detected exclusively in testis. Analysis of mRNA from testes of two germ cell deficient mutant strains did not reveal significant levels of meg1 transcripts. Analysis of RNA from enriched populations of spermatogenic cells from adult testes and localization by in situ hybridization revealed that meg1 transcripts are most abundant in pachytene spermatocytes. These results suggest a role for meg1 during germ cell differentiation, possibly during meiotic prophase.

Original languageEnglish
Pages (from-to)495-505
Number of pages11
JournalMolecular Cancer Research
Volume3
Issue number8
StatePublished - Aug 1992
Externally publishedYes

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