Hypoxia enhances transcytosis in intestinal enterocytes

D. V. Maltseva, M. Yu Shkurnikov, S. A. Nersisyan, S. V. Nikulin, A. A. Kurnosov, M. P. Raigorodskaya, A. I. Osipyants, E. A. Tonevitsky

Research output: Contribution to journalArticlepeer-review

Abstract

The integrity of the intestinal epithelial cell lining is crucial for the normal intestinal function. As a rule, intestinal inflammation is associated with additional tissue hypoxia, leading to the loss of epithelial monolayer integrity. However, in the absence of visible damage to the epithelium, there still might be a risk of infection driven by changes in the intracellular transport of bacteria-containing vesicles. The aim of this study was to investigate the effects of hypoxia on transcytosis using a human intestinal enterocyte model. We found that hypoxia enhances transcytosis of the model protein ricin 1.8-fold. The comparative transcriptome and proteome analyses revealed significant changes in the expression of genes involved in intracellular vesicle transport. Specifically, the expression of apoB (the regulator of lipid metabolism) was changed at both protein (6.5-fold) and mRNA (2.1-fold) levels. Further research is needed into the possible mechanism regulating gene expression in intestinal erythrocytes under hypoxic conditions.

Original languageEnglish
Pages (from-to)60-66
Number of pages7
JournalBulletin of Russian State Medical University
Issue number4
DOIs
StatePublished - Jul 2020
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2020 Pirogov Russian National Research Medical University. All rights reserved.

Keywords

  • Caco-2
  • Enterocyte
  • Hypoxia
  • Intestinal epithelium
  • MRNA
  • MicroRNA
  • Proteome
  • Transcriptome
  • Transcytosis

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