Abstract
Steroids with nonpolar side chains with 2, 4 and 8 C atoms were used as substrates for the 11α-hydroxylase of Rhizopus nigricans. Their bioconversion was compared to that of progesterone, which was found to be far the best substrate giving the highest total bioconversion. 3-keto-4-ene steroids with nonpolar side chains were converted to their hydroxylated products in a small yield or not at all. The absence of an oxygen function in the side chain did not affect the regio-specificity of the hydroxylation, but resulted in a much lower total bioconversion. The strong effect of the oxygen function and of the length of the side chain on hydroxylation with the 11α-hydroxylase of Rhizopus nigricans was demonstrated.
| Original language | English |
|---|---|
| Pages (from-to) | 287-288 |
| Number of pages | 2 |
| Journal | Journal of Steroid Biochemistry |
| Volume | 33 |
| Issue number | 2 |
| DOIs | |
| State | Published - Aug 1989 |
Bibliographical note
Funding Information:Acknowledgements-This work was supportedb y the ResearchC ommunityo f SloveniaT. he authorsw ish to thank Dr B. Kralj, “J. Stefan” Institute, Ljubljana, Yugoslavia,f or takingm asss pectraa nd M. Mart& for her skillful technicala ssistance.
Funding
Acknowledgements-This work was supportedb y the ResearchC ommunityo f SloveniaT. he authorsw ish to thank Dr B. Kralj, “J. Stefan” Institute, Ljubljana, Yugoslavia,f or takingm asss pectraa nd M. Mart& for her skillful technicala ssistance.
| Funders | Funder number |
|---|---|
| ResearchC ommunityo f SloveniaT |