Hydrogel-Inducing Graphene-Oxide-Derived Core–Shell Fiber Composite for Antibacterial Wound Dressing

Yuliya Kan, Julia V. Bondareva, Eugene S. Statnik, Elizaveta V. Koudan, Evgeniy V. Ippolitov, Mikhail S. Podporin, Polina A. Kovaleva, Roman R. Kapaev, Alexandra M. Gordeeva, Julijana Cvjetinovic, Dmitry A. Gorin, Stanislav A. Evlashin, Alexey I. Salimon, Fedor S. Senatov, Alexander M. Korsunsky

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

The study reveals the polymer–crosslinker interactions and functionality of hydrophilic nanofibers for antibacterial wound coatings. Coaxial electrospinning leverages a drug encapsulation protocol for a core–shell fiber composite with a core derived from polyvinyl alcohol and polyethylene glycol with amorphous silica (PVA-PEG-SiO2), and a shell originating from polyvinyl alcohol and graphene oxide (PVA-GO). Crosslinking with GO and SiO2 initiates the hydrogel transition for the fiber composite upon contact with moisture, which aims to optimize the drug release. The effect of hydrogel-inducing additives on the drug kinetics is evaluated in the case of chlorhexidine digluconate (CHX) encapsulation in the core of core–shell fiber composite PVA-PEG-SiO2-1x-CHX@PVA-GO. The release rate is assessed with the zero, first-order, Higuchi, and Korsmeyer–Peppas kinetic models, where the inclusion of crosslinking silica provides a longer degradation and release rate. CHX medicated core–shell composite provides sustainable antibacterial activity against Staphylococcus aureus.

Original languageEnglish
Article number6255
JournalInternational Journal of Molecular Sciences
Volume24
Issue number7
DOIs
StatePublished - 26 Mar 2023

Bibliographical note

Publisher Copyright:
© 2023 by the authors.

Funding

This work was supported by the Federal Academic Leadership program ’Priority 2030’ under the ‘Increase Competitiveness’ program of the National University of Science and Technology ‘MISIS’ (grant number K1-2022-032). The experiment was supported by Dr. M. S. Podporin and Prof. E. V. Ippolitov from Moscow State University of Medicine and Dentistry. The antibacterial activity of the CHX-loaded core–shell fiber mats was assessed for S. aureus cultivated on a solid medium agar plate. The working concentration of the suspension of bacteria corresponded to the 0.5 McFarland standard (∼1 × 10 colony forming units (CFU) mL). CHX-loaded core–shell fiber mats (PVA-PEG@PVA-GO-1x-CHX and PVA-PEG-SiO-1x-CHX@PVA-GO) and ‘empty’ PVA-PEG-SiO@PVA-GO fibers were sectioned into the disks with a diameter of 6 mm. The fiber samples and a cellulose control with Doxycycline were placed in the agar medium with the seeded colony of S. aureus. After static incubation at 37 °C for 1 day, the inhibition zones were photographed and measured to assess the bacterial sensitivity/resistivity parameter. −1 2 2 The experiment was supported by Dr. E. V. Koudan and Dr. F. S. Senatov from the National University of Science and Technology MISIS. Primary human fibroblasts passaged 3 times were cultured in DMEM containing 2 mM L-glutamine and an antimycotic–antibiotic solution (1x), with the addition of 10% (v/v) fetal bovine serum, at 37 °C and 5% CO. Then, the cells were transferred from the substrate with the use of a Versen solution and a 0.25% trypsin/EDTA solution. 2

FundersFunder number
Federal Academic Leadership
Moscow State University of Medicine and Dentistry
National University of Sciences and Technology
National University of Science and TechnologyK1-2022-032

    Keywords

    • crosslinking
    • graphene oxide
    • nanofiber
    • silica
    • wound dressing

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