Human muscle-derived, tissue specific, myocytotoxic T cell lines in dermatomyositis

U. Rosenschein; J. Radnay; D. Shoham; A. Shainberg; A. Klajman; L. A. Rozenszajn

Human muscle-derived, tissue specific, myocytotoxic T cell lines in dermatomyositis

U. Rosenschein
, J. Radnay
, D. Shoham
, A. Shainberg
, A. Klajman
, L. A. Rozenszajn

The Mina and Everard Goodman Faculty of Life Sciences - at Bar-Ilan University

Meir Hospital Sapir Medical Center

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Mononuclear cells were isolated from the inflamed muscle tissue of a patient suffering from dermatomyositis (DM). These were expanded in long-term culture and maintained in the presence of IL-2 containing culture medium. Two cell lines were established, one of the helper/inducer (OKT4⁺) and the other of the suppressor/cytotoxic phenotype (OKT8⁺). The OKT4⁺ cell line exhibited a non HLA-restricted, tissue-specific, myocytotoxic effect on rat muscle cell culture. Its lymphoproliferative response to human muscle antigen was HLA-restricted. The OKT8⁺ cell line exhibited a non HLA-restricted, tissue-specific response to muscle antigens and no myocytotoxic activity in in vitro rat muscle cell culture. It is likely that clones of OKT4⁺ lymphocytes in patients suffering from DM are associated with the pathogenesis of the disease - they probably mediate the diffuse damage to skeletal muscle through their myocytotoxic activity.

Original language	English
Pages (from-to)	309-318
Number of pages	10
Journal	Clinical and Experimental Immunology
Volume	67
Issue number	2
State	Published - Feb 1987

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Cite this

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title = "Human muscle-derived, tissue specific, myocytotoxic T cell lines in dermatomyositis",

abstract = "Mononuclear cells were isolated from the inflamed muscle tissue of a patient suffering from dermatomyositis (DM). These were expanded in long-term culture and maintained in the presence of IL-2 containing culture medium. Two cell lines were established, one of the helper/inducer (OKT4+) and the other of the suppressor/cytotoxic phenotype (OKT8+). The OKT4+ cell line exhibited a non HLA-restricted, tissue-specific, myocytotoxic effect on rat muscle cell culture. Its lymphoproliferative response to human muscle antigen was HLA-restricted. The OKT8+ cell line exhibited a non HLA-restricted, tissue-specific response to muscle antigens and no myocytotoxic activity in in vitro rat muscle cell culture. It is likely that clones of OKT4+ lymphocytes in patients suffering from DM are associated with the pathogenesis of the disease - they probably mediate the diffuse damage to skeletal muscle through their myocytotoxic activity.",

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T1 - Human muscle-derived, tissue specific, myocytotoxic T cell lines in dermatomyositis

AU - Rosenschein, U.

AU - Radnay, J.

AU - Shoham, D.

AU - Shainberg, A.

AU - Klajman, A.

AU - Rozenszajn, L. A.

PY - 1987/2

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N2 - Mononuclear cells were isolated from the inflamed muscle tissue of a patient suffering from dermatomyositis (DM). These were expanded in long-term culture and maintained in the presence of IL-2 containing culture medium. Two cell lines were established, one of the helper/inducer (OKT4+) and the other of the suppressor/cytotoxic phenotype (OKT8+). The OKT4+ cell line exhibited a non HLA-restricted, tissue-specific, myocytotoxic effect on rat muscle cell culture. Its lymphoproliferative response to human muscle antigen was HLA-restricted. The OKT8+ cell line exhibited a non HLA-restricted, tissue-specific response to muscle antigens and no myocytotoxic activity in in vitro rat muscle cell culture. It is likely that clones of OKT4+ lymphocytes in patients suffering from DM are associated with the pathogenesis of the disease - they probably mediate the diffuse damage to skeletal muscle through their myocytotoxic activity.

AB - Mononuclear cells were isolated from the inflamed muscle tissue of a patient suffering from dermatomyositis (DM). These were expanded in long-term culture and maintained in the presence of IL-2 containing culture medium. Two cell lines were established, one of the helper/inducer (OKT4+) and the other of the suppressor/cytotoxic phenotype (OKT8+). The OKT4+ cell line exhibited a non HLA-restricted, tissue-specific, myocytotoxic effect on rat muscle cell culture. Its lymphoproliferative response to human muscle antigen was HLA-restricted. The OKT8+ cell line exhibited a non HLA-restricted, tissue-specific response to muscle antigens and no myocytotoxic activity in in vitro rat muscle cell culture. It is likely that clones of OKT4+ lymphocytes in patients suffering from DM are associated with the pathogenesis of the disease - they probably mediate the diffuse damage to skeletal muscle through their myocytotoxic activity.

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JF - Clinical and Experimental Immunology

IS - 2

ER -

Human muscle-derived, tissue specific, myocytotoxic T cell lines in dermatomyositis

Abstract

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