Human muscle-derived, tissue specific, myocytotoxic T cell lines in dermatomyositis

U. Rosenschein, J. Radnay, D. Shoham, A. Shainberg, A. Klajman, L. A. Rozenszajn

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Mononuclear cells were isolated from the inflamed muscle tissue of a patient suffering from dermatomyositis (DM). These were expanded in long-term culture and maintained in the presence of IL-2 containing culture medium. Two cell lines were established, one of the helper/inducer (OKT4+) and the other of the suppressor/cytotoxic phenotype (OKT8+). The OKT4+ cell line exhibited a non HLA-restricted, tissue-specific, myocytotoxic effect on rat muscle cell culture. Its lymphoproliferative response to human muscle antigen was HLA-restricted. The OKT8+ cell line exhibited a non HLA-restricted, tissue-specific response to muscle antigens and no myocytotoxic activity in in vitro rat muscle cell culture. It is likely that clones of OKT4+ lymphocytes in patients suffering from DM are associated with the pathogenesis of the disease - they probably mediate the diffuse damage to skeletal muscle through their myocytotoxic activity.

Original languageEnglish
Pages (from-to)309-318
Number of pages10
JournalClinical and Experimental Immunology
Volume67
Issue number2
StatePublished - Feb 1987

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