Human Endothelial and Osteoblast Co-cultures on 3D Biomaterials

Ronald E. Unger, Sven Halstenberg, Anne Sartoris, C. James Kirkpatrick

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

16 Scopus citations


Increasingly, in vitro experiments are being used to evaluate the cell compatibility of novel biomaterials. Single cell cultures have been used to determine how well cells attach, grow, and exhibit characteristic functions on these materials and the outcome of such tests is generally accepted as an indicator of biocompatibility. However, organs and tissues are not made up of one cell type and the interaction of cells is known to be an essential factor for physiological cell function. To more accurately examine biomaterials for bone regeneration, we have developed methods to coculture osteoblasts, which are the primary cell type making up bone, and endothelial cells, which form the vasculature supplying cells in the bone with oxygen and nutrients to survive on 2- and 3-D biomaterials.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages13
StatePublished - 2011
Externally publishedYes

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Bibliographical note

Publisher Copyright:
© 2011, Springer Science+Business Media, LLC.


  • Biomaterials
  • Bone
  • Coculture
  • Endothelial cell
  • Human
  • Osteoblast
  • Tissue regeneration


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