High-throughput single-molecule optofluidic analysis

Soohong Kim; Aaron M. Streets; Ron R. Lin; Stephen R. Quake; Shimon Weiss; Devdoot S. Majumdar

doi:10.1038/nmeth.1569

High-throughput single-molecule optofluidic analysis

Soohong Kim, Aaron M. Streets, Ron R. Lin, Stephen R. Quake, Shimon Weiss, Devdoot S. Majumdar

Research output: Contribution to journal › Article › peer-review

74 Scopus citations

Abstract

We describe a high-throughput, automated single-molecule measurement system, equipped with microfluidics. The microfluidic mixing device has integrated valves and pumps to accurately accomplish titration of biomolecules with picoliter resolution. We demonstrate that the approach enabled rapid sampling of biomolecule conformational landscape and of enzymatic activity, in the form of transcription by Escherichia coli RNA polymerase, as a function of the chemical environment.

Original language	English
Pages (from-to)	242-245
Number of pages	4
Journal	Nature Methods
Volume	8
Issue number	3
DOIs	https://doi.org/10.1038/nmeth.1569
State	Published - Mar 2011
Externally published	Yes

Bibliographical note

Funding Information:
We thank R. Colyer, P. Blainey and other members of the Weiss and Quake laboratories for helpful discussions. This work was supported by US National Science Foundation Frontiers in Integrative Biological Research grant 0623664 and National Institutes of Health grant GM069709. Fluorescence spectroscopy was performed at the University of California, Los Angeles and California NanoSystems Institute Advanced Light Microscopy and Spectroscopy Shared Facility. A.M.S. was supported by the Stanford University Diversifying Academia, Recruiting Excellence fellowship.

Funding

We thank R. Colyer, P. Blainey and other members of the Weiss and Quake laboratories for helpful discussions. This work was supported by US National Science Foundation Frontiers in Integrative Biological Research grant 0623664 and National Institutes of Health grant GM069709. Fluorescence spectroscopy was performed at the University of California, Los Angeles and California NanoSystems Institute Advanced Light Microscopy and Spectroscopy Shared Facility. A.M.S. was supported by the Stanford University Diversifying Academia, Recruiting Excellence fellowship.

Funders	Funder number
US National Science Foundation	0623664
National Institutes of Health	GM069709
NIH Office of the Director	DP1OD000251
Stanford University

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abstract = "We describe a high-throughput, automated single-molecule measurement system, equipped with microfluidics. The microfluidic mixing device has integrated valves and pumps to accurately accomplish titration of biomolecules with picoliter resolution. We demonstrate that the approach enabled rapid sampling of biomolecule conformational landscape and of enzymatic activity, in the form of transcription by Escherichia coli RNA polymerase, as a function of the chemical environment.",

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AU - Weiss, Shimon

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High-throughput single-molecule optofluidic analysis

Abstract

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