High-throughput FCS using an LCOS spatial light modulator and an 8× 1 SPAD array

RA Colyer; G Scalia; I Rech; A Gulinatti; M Ghioni; S Cova; S. Weiss; X Michalet

High-throughput FCS using an LCOS spatial light modulator and an 8× 1 SPAD array

RA Colyer, G Scalia, I Rech, A Gulinatti, M Ghioni, S Cova, S. Weiss, X Michalet

Department of Physics

Research output: Contribution to journal › Article › peer-review

Abstract

We present a novel approach to high-throughput Fluorescence Correlation Spectroscopy (FCS) which enables us to obtain one order of magnitude improvement in acquisition time. Our approach utilizes a liquid crystal on silicon spatial light modulator to generate dynamically adjustable focal spots, and uses an eight-pixel monolithic single-photon avalanche photodiode array. We demonstrate the capabilities of this system by showing FCS of Rhodamine 6G under various viscosities, and by showing that, with proper calibration of each detection channel, one order of magnitude improvement in acquisition speed is obtained. More generally, our approach will allow higher throughput single-molecule studies to be performed.

Original language	American English
Pages (from-to)	1408-1431
Journal	Biomedical Optics Express
Volume	1
Issue number	5
State	Published - 2010

Cite this

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title = "High-throughput FCS using an LCOS spatial light modulator and an 8× 1 SPAD array",

abstract = "We present a novel approach to high-throughput Fluorescence Correlation Spectroscopy (FCS) which enables us to obtain one order of magnitude improvement in acquisition time. Our approach utilizes a liquid crystal on silicon spatial light modulator to generate dynamically adjustable focal spots, and uses an eight-pixel monolithic single-photon avalanche photodiode array. We demonstrate the capabilities of this system by showing FCS of Rhodamine 6G under various viscosities, and by showing that, with proper calibration of each detection channel, one order of magnitude improvement in acquisition speed is obtained. More generally, our approach will allow higher throughput single-molecule studies to be performed.",

author = "RA Colyer and G Scalia and I Rech and A Gulinatti and M Ghioni and S Cova and S. Weiss and X Michalet",

year = "2010",

language = "American English",

volume = "1",

pages = "1408--1431",

journal = "Biomedical Optics Express",

issn = "2156-7085",

publisher = "Optica Publishing Group (formerly OSA)",

number = "5",

}

TY - JOUR

T1 - High-throughput FCS using an LCOS spatial light modulator and an 8× 1 SPAD array

AU - Colyer, RA

AU - Scalia, G

AU - Rech, I

AU - Gulinatti, A

AU - Ghioni, M

AU - Cova, S

AU - Weiss, S.

AU - Michalet, X

PY - 2010

Y1 - 2010

N2 - We present a novel approach to high-throughput Fluorescence Correlation Spectroscopy (FCS) which enables us to obtain one order of magnitude improvement in acquisition time. Our approach utilizes a liquid crystal on silicon spatial light modulator to generate dynamically adjustable focal spots, and uses an eight-pixel monolithic single-photon avalanche photodiode array. We demonstrate the capabilities of this system by showing FCS of Rhodamine 6G under various viscosities, and by showing that, with proper calibration of each detection channel, one order of magnitude improvement in acquisition speed is obtained. More generally, our approach will allow higher throughput single-molecule studies to be performed.

AB - We present a novel approach to high-throughput Fluorescence Correlation Spectroscopy (FCS) which enables us to obtain one order of magnitude improvement in acquisition time. Our approach utilizes a liquid crystal on silicon spatial light modulator to generate dynamically adjustable focal spots, and uses an eight-pixel monolithic single-photon avalanche photodiode array. We demonstrate the capabilities of this system by showing FCS of Rhodamine 6G under various viscosities, and by showing that, with proper calibration of each detection channel, one order of magnitude improvement in acquisition speed is obtained. More generally, our approach will allow higher throughput single-molecule studies to be performed.

UR - http://www.opticsinfobase.org/abstract.cfm?URI=boe-1-5-1408

M3 - Article

SN - 2156-7085

VL - 1

SP - 1408

EP - 1431

JO - Biomedical Optics Express

JF - Biomedical Optics Express

IS - 5

ER -

High-throughput FCS using an LCOS spatial light modulator and an 8× 1 SPAD array

Abstract

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