High speed, inverted optical-resolution photoacoustic microscopy

Bin Rao; Konstantin Maslov; Amos Danielli; Ruiming Chen; K. Kirk Shung; Qifa Zhou; Lihong V. Wang

doi:10.1117/12.873568

High speed, inverted optical-resolution photoacoustic microscopy

Bin Rao, Konstantin Maslov, Amos Danielli, Ruiming Chen, K. Kirk Shung, Qifa Zhou, Lihong V. Wang

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

Photoacoustic microscopy (PAM) offers label-free, optical absorption contrast. A high-speed, high-resolution PAM system in an inverted microscope configuration with a laser pulse repetition rate of 100,000 Hz and a stationary ultrasonic transducer was built. Four-dimensional in vivo imaging of microcirculation in mouse skin was achieved at 18 three-dimensional volumes per second with repeated two-dimensional raster scans of 100 by 50 points. The corresponding twodimensional B-scan (50 A-lines) frame rate was 1800 Hz, and the one-dimensional A-scan rate was 90,000 Hz. The lateral resolution is 0.23±0.03 μm for Au nano-wire imaging, which is 2.0 times below the diffraction limit.

Original language	English
Title of host publication	Photons Plus Ultrasound
Subtitle of host publication	Imaging and Sensing 2011
DOIs	https://doi.org/10.1117/12.873568
State	Published - 2011
Externally published	Yes
Event	Photons Plus Ultrasound: Imaging and Sensing 2011 - San Francisco, CA, United States Duration: 23 Jan 2011 → 25 Jan 2011

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	7899
ISSN (Print)	1605-7422

Conference

Conference	Photons Plus Ultrasound: Imaging and Sensing 2011
Country/Territory	United States
City	San Francisco, CA
Period	23/01/11 → 25/01/11

Access to Document

10.1117/12.873568

Cite this

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title = "High speed, inverted optical-resolution photoacoustic microscopy",

abstract = "Photoacoustic microscopy (PAM) offers label-free, optical absorption contrast. A high-speed, high-resolution PAM system in an inverted microscope configuration with a laser pulse repetition rate of 100,000 Hz and a stationary ultrasonic transducer was built. Four-dimensional in vivo imaging of microcirculation in mouse skin was achieved at 18 three-dimensional volumes per second with repeated two-dimensional raster scans of 100 by 50 points. The corresponding twodimensional B-scan (50 A-lines) frame rate was 1800 Hz, and the one-dimensional A-scan rate was 90,000 Hz. The lateral resolution is 0.23±0.03 μm for Au nano-wire imaging, which is 2.0 times below the diffraction limit.",

author = "Bin Rao and Konstantin Maslov and Amos Danielli and Ruiming Chen and Shung, {K. Kirk} and Qifa Zhou and Wang, {Lihong V.}",

year = "2011",

doi = "10.1117/12.873568",

language = "אנגלית",

isbn = "9780819484369",

series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

booktitle = "Photons Plus Ultrasound",

note = "Photons Plus Ultrasound: Imaging and Sensing 2011 ; Conference date: 23-01-2011 Through 25-01-2011",

}

Rao, B, Maslov, K, Danielli, A, Chen, R, Shung, KK, Zhou, Q & Wang, LV 2011, High speed, inverted optical-resolution photoacoustic microscopy. in Photons Plus Ultrasound: Imaging and Sensing 2011., 78990T, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 7899, Photons Plus Ultrasound: Imaging and Sensing 2011, San Francisco, CA, United States, 23/01/11. https://doi.org/10.1117/12.873568

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AU - Zhou, Qifa

AU - Wang, Lihong V.

PY - 2011

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AB - Photoacoustic microscopy (PAM) offers label-free, optical absorption contrast. A high-speed, high-resolution PAM system in an inverted microscope configuration with a laser pulse repetition rate of 100,000 Hz and a stationary ultrasonic transducer was built. Four-dimensional in vivo imaging of microcirculation in mouse skin was achieved at 18 three-dimensional volumes per second with repeated two-dimensional raster scans of 100 by 50 points. The corresponding twodimensional B-scan (50 A-lines) frame rate was 1800 Hz, and the one-dimensional A-scan rate was 90,000 Hz. The lateral resolution is 0.23±0.03 μm for Au nano-wire imaging, which is 2.0 times below the diffraction limit.

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ER -

High speed, inverted optical-resolution photoacoustic microscopy

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