High Spatio-Temporal Resolution Condenser-Free Quantitative Phase Contrast Microscopy

Ying Ma; Lin Ma; Juanjuan Zheng; Min Liu; Zeev Zalevsky; Peng Gao

doi:10.3389/fphy.2022.892529

High Spatio-Temporal Resolution Condenser-Free Quantitative Phase Contrast Microscopy

Ying Ma, Lin Ma, Juanjuan Zheng, Min Liu, Zeev Zalevsky, Peng Gao

Bar-Ilan University - The Alexander Kofkin Faculty of Engineering

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Quantitative phase-contrast microscopy (QPCM) provides an effective approach for label-free detection of transparent samples. In this study, we propose a condenser-free quantitative phase-contrast microscopy (CF-QPCM), in which several light-emitting diodes (LEDs) distributed on a ring are used for direct ultra-oblique illumination. Such condenser-free design greatly simplifies the system’s structure and releases the space for installing samples. Quantitative phase maps are reconstructed by retarding the unscattered components of the object waves for a series of phases 0, π/2, π, and 3π/2 through a high-speed spatial light modulator (SLM). With this system, quantitative phase imaging of live cells has been achieved at a spatial resolution of 231 nm (lateral) and a frame rate of 250 Hz. We believe that the proposed CF-QPCM can contribute to biomedical, industrial, chemistry fields, etc.

Original language	English
Article number	892529
Journal	Frontiers in Physics
Volume	10
DOIs	https://doi.org/10.3389/fphy.2022.892529
State	Published - 9 May 2022

Bibliographical note

Publisher Copyright:
Copyright © 2022 Ma, Ma, Zheng, Liu, Zalevsky and Gao.

Funding

The authors acknowledge the support from the National Natural Science Foundation of China (NSFC 62105251, 62075177, 12104354); Natural Science Foundation of Shaanxi Province (2021JQ-184); Fundamental Research Funds for the Central Universities (XJS210503 and XJS210504); the National Key Research and Development Program of China (2021YFF0700303); Exchange Program Between China-and Poland (2021–2022); Guangdong Basic and Applied Basic Research Foundation (2020A1515110590); Research Fund of State Key Laboratory of Transient Optics and Photonics.

Funders	Funder number
National Natural Science Foundation of China	62075177, 12104354, 62105251
Natural Science Foundation of Shaanxi Province	2021JQ-184
State Key Laboratory of Transient Optics and Photonics
National Key Research and Development Program of China	2021YFF0700303
Fundamental Research Funds for the Central Universities	XJS210504, XJS210503
Basic and Applied Basic Research Foundation of Guangdong Province	2020A1515110590

Keywords

label-free
non-invasive
phase contrast
quantitative phase imaging
ultra-oblique illumination

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10.3389/fphy.2022.892529

Cite this

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title = "High Spatio-Temporal Resolution Condenser-Free Quantitative Phase Contrast Microscopy",

abstract = "Quantitative phase-contrast microscopy (QPCM) provides an effective approach for label-free detection of transparent samples. In this study, we propose a condenser-free quantitative phase-contrast microscopy (CF-QPCM), in which several light-emitting diodes (LEDs) distributed on a ring are used for direct ultra-oblique illumination. Such condenser-free design greatly simplifies the system{\textquoteright}s structure and releases the space for installing samples. Quantitative phase maps are reconstructed by retarding the unscattered components of the object waves for a series of phases 0, π/2, π, and 3π/2 through a high-speed spatial light modulator (SLM). With this system, quantitative phase imaging of live cells has been achieved at a spatial resolution of 231 nm (lateral) and a frame rate of 250 Hz. We believe that the proposed CF-QPCM can contribute to biomedical, industrial, chemistry fields, etc.",

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PY - 2022/5/9

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High Spatio-Temporal Resolution Condenser-Free Quantitative Phase Contrast Microscopy

Abstract

Bibliographical note

Funding

Keywords

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