High-Resolution Imaging and Analysis of Individual Nuclear Pore Complexes

Boris Fichtman; Saroj G. Regmi; Mary Dasso; Amnon Harel

doi:10.1007/978-1-0716-2337-4_29

High-Resolution Imaging and Analysis of Individual Nuclear Pore Complexes

Boris Fichtman, Saroj G. Regmi, Mary Dasso, Amnon Harel

Bar-Ilan University - Azrieli Faculty of Medicine

National Institutes of Health

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

3 Scopus citations

Abstract

Field emission scanning electron microscopy (FESEM) is a well-established technique for acquiring three-dimensional surface images of nuclear pore complexes (NPCs). We present an optimized protocol for the exposure of mammalian cell nuclei and direct surface imaging of nuclear envelopes by FESEM, allowing for a detailed morphological comparison of individual NPCs, without the need for averaging techniques. This provides a unique high resolution tool for studying the effects of cellular stress, specific genetic manipulations and inherited diseases on the ultrastructure of human NPCs.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	461-471
Number of pages	11
DOIs	https://doi.org/10.1007/978-1-0716-2337-4_29
State	Published - 2022

Publication series

Name	Methods in Molecular Biology
Volume	2502
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Bibliographical note

Publisher Copyright:
© 2022, Springer Science+Business Media, LLC, part of Springer Nature.

Funding

This work was supported by research grants from the Israel Science Foundation (958/15) and the Christians for Israel Chair in Medical Research to A.H.

Funders	Funder number
Christians for Israel Chair in Medical Research
Eunice Kennedy Shriver National Institute of Child Health and Human Development	ZIAHD008954
Israel Science Foundation	958/15

Keywords

Auxin-inducible degron
Central channel particles
Mammalian cell culture
Nuclear envelope
Nuclear pore complex
Scanning electron microscopy

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/978-1-0716-2337-4_29

Cite this

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High-Resolution Imaging and Analysis of Individual Nuclear Pore Complexes

Abstract

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Bibliographical note

Funding

Keywords

UN SDGs

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