High energy carbon ion beam irradiated sensor for the voltammetric sensing of purine nucleosides of DNA

Rosy; Pankaj Gupta; Rajendra N. Goyal

doi:10.1016/j.snb.2015.10.006

High energy carbon ion beam irradiated sensor for the voltammetric sensing of purine nucleosides of DNA

Rosy, Pankaj Gupta, Rajendra N. Goyal

Indian Institute of Technology Roorkee

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

The effect of high energy carbon ion beam as the modification tool for the multiwalled carbon nanotubes (MWCNT) has been studied. The change in the morphology, length and diameter of the carbon nanotubes upon irradiation is analyzed using Raman spectroscopy, atomic force microscopy and field emission scanning electron microscopy. The irradiated MWCNTs had a diameter in the range of 3-27 nm which is much lower in comparison to the diameter of the tubes present in the pristine state. The irradiated MWCNT modified glassy carbon has been further used as a sensor for the electrochemical investigation of deoxyguanosine and deoxyadenosine. A detection limit of 507 and 300 nM was observed for deoxyguanosine and deoxyadenosine respectively. The simultaneous determination of the two analytes was performed using the fabricated sensor and its practical utility was tested by analyzing DNA extracted from herring sperm and MCF7 cell line for the presence of deoxyguanosine and deoxyadenosine. The common biomolecules present in blood and urine did not interfere in the determination and a high stability and reproducibility of the sensor was observed.

Original language	English
Pages (from-to)	884-892
Number of pages	9
Journal	Sensors and Actuators, B: Chemical
Volume	223
DOIs	https://doi.org/10.1016/j.snb.2015.10.006
State	Published - 1 Feb 2016
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2015 Elsevier B.V. All rights reserved.

Funding

Authors (Rosy and PG) are thankful to the Ministry of Human Resources Development, New Delhi for the award of Senior Research Fellowship. Thanks are also due to the Director, Inter University Accelerator Center (IUAC), New Delhi for permitting irradiation experiments and to Dr. Fouran Singh, Scientist F, Material Science Group, IUAC for helpful discussions.

Funders	Funder number
Ministry of Education and Human Resources Development

Keywords

Deoxyadenosine
Deoxyguanosine
Glassy carbon electrode
Ion beam
Irradiation
Multiwalled carbon nanotubes
Voltammetry

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10.1016/j.snb.2015.10.006

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title = "High energy carbon ion beam irradiated sensor for the voltammetric sensing of purine nucleosides of DNA",

abstract = "The effect of high energy carbon ion beam as the modification tool for the multiwalled carbon nanotubes (MWCNT) has been studied. The change in the morphology, length and diameter of the carbon nanotubes upon irradiation is analyzed using Raman spectroscopy, atomic force microscopy and field emission scanning electron microscopy. The irradiated MWCNTs had a diameter in the range of 3-27 nm which is much lower in comparison to the diameter of the tubes present in the pristine state. The irradiated MWCNT modified glassy carbon has been further used as a sensor for the electrochemical investigation of deoxyguanosine and deoxyadenosine. A detection limit of 507 and 300 nM was observed for deoxyguanosine and deoxyadenosine respectively. The simultaneous determination of the two analytes was performed using the fabricated sensor and its practical utility was tested by analyzing DNA extracted from herring sperm and MCF7 cell line for the presence of deoxyguanosine and deoxyadenosine. The common biomolecules present in blood and urine did not interfere in the determination and a high stability and reproducibility of the sensor was observed.",

keywords = "Deoxyadenosine, Deoxyguanosine, Glassy carbon electrode, Ion beam, Irradiation, Multiwalled carbon nanotubes, Voltammetry",

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AU - Goyal, Rajendra N.

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N2 - The effect of high energy carbon ion beam as the modification tool for the multiwalled carbon nanotubes (MWCNT) has been studied. The change in the morphology, length and diameter of the carbon nanotubes upon irradiation is analyzed using Raman spectroscopy, atomic force microscopy and field emission scanning electron microscopy. The irradiated MWCNTs had a diameter in the range of 3-27 nm which is much lower in comparison to the diameter of the tubes present in the pristine state. The irradiated MWCNT modified glassy carbon has been further used as a sensor for the electrochemical investigation of deoxyguanosine and deoxyadenosine. A detection limit of 507 and 300 nM was observed for deoxyguanosine and deoxyadenosine respectively. The simultaneous determination of the two analytes was performed using the fabricated sensor and its practical utility was tested by analyzing DNA extracted from herring sperm and MCF7 cell line for the presence of deoxyguanosine and deoxyadenosine. The common biomolecules present in blood and urine did not interfere in the determination and a high stability and reproducibility of the sensor was observed.

AB - The effect of high energy carbon ion beam as the modification tool for the multiwalled carbon nanotubes (MWCNT) has been studied. The change in the morphology, length and diameter of the carbon nanotubes upon irradiation is analyzed using Raman spectroscopy, atomic force microscopy and field emission scanning electron microscopy. The irradiated MWCNTs had a diameter in the range of 3-27 nm which is much lower in comparison to the diameter of the tubes present in the pristine state. The irradiated MWCNT modified glassy carbon has been further used as a sensor for the electrochemical investigation of deoxyguanosine and deoxyadenosine. A detection limit of 507 and 300 nM was observed for deoxyguanosine and deoxyadenosine respectively. The simultaneous determination of the two analytes was performed using the fabricated sensor and its practical utility was tested by analyzing DNA extracted from herring sperm and MCF7 cell line for the presence of deoxyguanosine and deoxyadenosine. The common biomolecules present in blood and urine did not interfere in the determination and a high stability and reproducibility of the sensor was observed.

KW - Deoxyadenosine

KW - Deoxyguanosine

KW - Glassy carbon electrode

KW - Ion beam

KW - Irradiation

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KW - Voltammetry

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High energy carbon ion beam irradiated sensor for the voltammetric sensing of purine nucleosides of DNA

Abstract

Bibliographical note

Funding

Keywords

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