High affinity scFv-hapten pair as a tool for quantum dot labeling and tracking of single proteins in live cells

Gopal Iyer, Xavier Michalet, Yun Pei Chang, Fabien F. Pinaud, Stephanie E. Matyas, Gregory Payne, Shimon Weiss

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

We describe a general approach to label cell surface proteins using quantum dots (QD) for single-molecule tracking. QDs coated with small-hapten modified peptides are targeted to cell surface fusion proteins containing the corresponding single-chain fragment antibody (scFv). The approach Is illustrated with the small hapten fluorescein (FL) and a high-affinity anti-FL scFv fused to two different proteins in yeast and murlne neuronal cell line N2a.

Original languageEnglish
Pages (from-to)4618-4623
Number of pages6
JournalNano Letters
Volume8
Issue number12
DOIs
StatePublished - Dec 2008
Externally publishedYes

Funding

FundersFunder number
National Cancer InstituteP30CA016042
National Institute of Allergy and Infectious DiseasesP30AI028697
National Institute of Biomedical Imaging and BioengineeringR01EB000312

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