High affinity scFv-hapten pair as a tool for quantum dot labeling and tracking of single proteins in live cells

Gopal Iyer; Xavier Michalet; Yun Pei Chang; Fabien F. Pinaud; Stephanie E. Matyas; Gregory Payne; Shimon Weiss

doi:10.1021/nl8032284

High affinity scFv-hapten pair as a tool for quantum dot labeling and tracking of single proteins in live cells

Gopal Iyer, Xavier Michalet, Yun Pei Chang, Fabien F. Pinaud, Stephanie E. Matyas, Gregory Payne, Shimon Weiss

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

We describe a general approach to label cell surface proteins using quantum dots (QD) for single-molecule tracking. QDs coated with small-hapten modified peptides are targeted to cell surface fusion proteins containing the corresponding single-chain fragment antibody (scFv). The approach Is illustrated with the small hapten fluorescein (FL) and a high-affinity anti-FL scFv fused to two different proteins in yeast and murlne neuronal cell line N2a.

Original language	English
Pages (from-to)	4618-4623
Number of pages	6
Journal	Nano Letters
Volume	8
Issue number	12
DOIs	https://doi.org/10.1021/nl8032284
State	Published - Dec 2008
Externally published	Yes

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10.1021/nl8032284

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abstract = "We describe a general approach to label cell surface proteins using quantum dots (QD) for single-molecule tracking. QDs coated with small-hapten modified peptides are targeted to cell surface fusion proteins containing the corresponding single-chain fragment antibody (scFv). The approach Is illustrated with the small hapten fluorescein (FL) and a high-affinity anti-FL scFv fused to two different proteins in yeast and murlne neuronal cell line N2a.",

author = "Gopal Iyer and Xavier Michalet and Chang, {Yun Pei} and Pinaud, {Fabien F.} and Matyas, {Stephanie E.} and Gregory Payne and Shimon Weiss",

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AU - Iyer, Gopal

AU - Michalet, Xavier

AU - Chang, Yun Pei

AU - Pinaud, Fabien F.

AU - Matyas, Stephanie E.

AU - Payne, Gregory

AU - Weiss, Shimon

PY - 2008/12

Y1 - 2008/12

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AB - We describe a general approach to label cell surface proteins using quantum dots (QD) for single-molecule tracking. QDs coated with small-hapten modified peptides are targeted to cell surface fusion proteins containing the corresponding single-chain fragment antibody (scFv). The approach Is illustrated with the small hapten fluorescein (FL) and a high-affinity anti-FL scFv fused to two different proteins in yeast and murlne neuronal cell line N2a.

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ER -

High affinity scFv-hapten pair as a tool for quantum dot labeling and tracking of single proteins in live cells

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