High Affinity scFv− Hapten Pair as a Tool for Quantum Dot Labeling and Tracking of Single Proteins in Live Cells

G Iyer; X Michalet; YP Chang; FF Pinaud; SE Matyas; G Payne; S. Weiss

High Affinity scFv− Hapten Pair as a Tool for Quantum Dot Labeling and Tracking of Single Proteins in Live Cells

G Iyer, X Michalet, YP Chang, FF Pinaud, SE Matyas, G Payne, S. Weiss

Department of Physics

Research output: Contribution to journal › Article › peer-review

Abstract

We describe a general approach to label cell surface proteins using quantum dots (QD) for single-molecule tracking. QDs coated with small-hapten modified peptides are targeted to cell surface fusion proteins containing the corresponding single-chain fragment antibody (scFv). The approach is illustrated with the small hapten fluorescein (FL) and a high-affinity anti-FL scFv fused to two different proteins in yeast and murine neuronal cell line N2a.

Original language	American English
Pages (from-to)	4618-4623
Journal	Nano Letters
Volume	8
Issue number	12
State	Published - 2008

Cite this

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title = "High Affinity scFv− Hapten Pair as a Tool for Quantum Dot Labeling and Tracking of Single Proteins in Live Cells",

abstract = "We describe a general approach to label cell surface proteins using quantum dots (QD) for single-molecule tracking. QDs coated with small-hapten modified peptides are targeted to cell surface fusion proteins containing the corresponding single-chain fragment antibody (scFv). The approach is illustrated with the small hapten fluorescein (FL) and a high-affinity anti-FL scFv fused to two different proteins in yeast and murine neuronal cell line N2a.",

author = "G Iyer and X Michalet and YP Chang and FF Pinaud and SE Matyas and G Payne and S. Weiss",

year = "2008",

language = "American English",

volume = "8",

pages = "4618--4623",

journal = "Nano Letters",

issn = "1530-6984",

publisher = "American Chemical Society",

number = "12",

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TY - JOUR

T1 - High Affinity scFv− Hapten Pair as a Tool for Quantum Dot Labeling and Tracking of Single Proteins in Live Cells

AU - Iyer, G

AU - Michalet, X

AU - Chang, YP

AU - Pinaud, FF

AU - Matyas, SE

AU - Payne, G

AU - Weiss, S.

PY - 2008

Y1 - 2008

N2 - We describe a general approach to label cell surface proteins using quantum dots (QD) for single-molecule tracking. QDs coated with small-hapten modified peptides are targeted to cell surface fusion proteins containing the corresponding single-chain fragment antibody (scFv). The approach is illustrated with the small hapten fluorescein (FL) and a high-affinity anti-FL scFv fused to two different proteins in yeast and murine neuronal cell line N2a.

AB - We describe a general approach to label cell surface proteins using quantum dots (QD) for single-molecule tracking. QDs coated with small-hapten modified peptides are targeted to cell surface fusion proteins containing the corresponding single-chain fragment antibody (scFv). The approach is illustrated with the small hapten fluorescein (FL) and a high-affinity anti-FL scFv fused to two different proteins in yeast and murine neuronal cell line N2a.

UR - http://pubs.acs.org/doi/pdf/10.1021/nl8032284

M3 - Article

SN - 1530-6984

VL - 8

SP - 4618

EP - 4623

JO - Nano Letters

JF - Nano Letters

IS - 12

ER -

High Affinity scFv− Hapten Pair as a Tool for Quantum Dot Labeling and Tracking of Single Proteins in Live Cells

Abstract

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