Peripheral blood leukocytes (PBL) of carp respond in vitro to a variety of phytomitogens, shown to be T-cell specific or B-cell specific in mammalian systems. Some basic differences have been observed in the proliferative response of carp PBL to PHA (phytohemagglutinin), ConA (concanavalin A) and LPS (lipopolysaccharide): (1) The response to PHA and ConA was found to be highly dependent upon the continuous presence of mitogen in the medium, in contrast to LPS, where after the initial stimulation, cells could continue to proliferate for several days without mitogen. (2) Lymphoblasts grown in long term culture with either PHA or Con A could be transferred into medium containing the other mitogen without impairing cell proliferation, but cell growth was reduced to background level following transfer into LPS-containing medium. LPS grown cells continue to proliferate independently of the mitogen content of the medium. (3) Co-stimulation with LPS+PHA or LPS+ConA results in a synergistic response, while constimulation with PHA+ConA results in inhibition of DNA synthesis. (4) Several morphological differences have been observed between cells proliferating in the presence of PHA and those proliferating in the presence of LPS. It is suggested that while the PHA and ConA responsive cells may belong to the same lymphocyte subpopulation, they are distinct from the LPS-responsive subpopulation.
|Number of pages||10|
|Journal||Developmental and Comparative Immunology|
|State||Published - 1984|
Bibliographical noteFunding Information:
Acknowled~ements~ Supported by research grant from the National Council for Research and Development, Israel, and the KGSS Geesthacht-Tesperhude, Germany, and by Grant No. 01-434 from Bar-Ilan University. We wish to thank Mr. J. Muravich for his photographic work, and Mrs. Bluma Lederhendler for typing this manuscript.