Hepatitis E virus ORF1 encoded non structural protein-host protein interaction network

Nishant Kumar Ojha; Kavita S. Lole

doi:10.1016/j.virusres.2015.12.007

Hepatitis E virus ORF1 encoded non structural protein-host protein interaction network

Nishant Kumar Ojha
, Kavita S. Lole

Indian Council of Medical Research

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

Hepatitis E virus ORF1 encoded non-structural polyprotein (nsP) consist of multiple domains, namely: Methyltransferase, Y-domain, Protease, X-domain, Helicase and RNA dependent RNA polymerase. We have attempted to identify human liver cell proteins that are interacting with HEV ORF1 encoded functional domains by using Y2H screening. A total of 155 protein-protein interactions between HEV-ORF1 and human proteins were identified. Comparative analysis of the HEV-ORF1-Human interaction network with reconstructed human interactome showed that the cellular proteins interacting with HEV-ORF1 are central and interconnected. Enrichment analysis of Gene Ontology and cellular pathways showed that the viral proteins preferentially interacted with the proteins of metabolism and energy generation along with host immune response and ubiquitin proteasomal pathways. The mTOR and focal adhesion pathways were also targeted by the virus. These interactions suggest that HEV probably utilizes important proteins in carbohydrate metabolism, energy generation and iron homoeostasis in the host cells during its establishment.

Original language	English
Pages (from-to)	195-204
Number of pages	10
Journal	Virus Research
Volume	213
DOIs	https://doi.org/10.1016/j.virusres.2015.12.007
State	Published - 2 Feb 2016
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2015 Elsevier B.V.

Funding

Funding to carry out this research was provided by the intramural grant of the National Institute of Virology, Pune (Grant ID: HEP 1104 ). N.K.O. received graduate research fellowship from the Council for Scientific and Industrial Research, Government of India (Grant ID: 09/698[0015]/2009-EMR-1 ).

Funders	Funder number
National Institute of Virology	HEP 1104
Council of Scientific and Industrial Research, India	09/698[0015]/2009-EMR-1

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Gene Ontology
Virus-host interaction
Yeast two hybrid

Access to Document

10.1016/j.virusres.2015.12.007

Cite this

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title = "Hepatitis E virus ORF1 encoded non structural protein-host protein interaction network",

abstract = "Hepatitis E virus ORF1 encoded non-structural polyprotein (nsP) consist of multiple domains, namely: Methyltransferase, Y-domain, Protease, X-domain, Helicase and RNA dependent RNA polymerase. We have attempted to identify human liver cell proteins that are interacting with HEV ORF1 encoded functional domains by using Y2H screening. A total of 155 protein-protein interactions between HEV-ORF1 and human proteins were identified. Comparative analysis of the HEV-ORF1-Human interaction network with reconstructed human interactome showed that the cellular proteins interacting with HEV-ORF1 are central and interconnected. Enrichment analysis of Gene Ontology and cellular pathways showed that the viral proteins preferentially interacted with the proteins of metabolism and energy generation along with host immune response and ubiquitin proteasomal pathways. The mTOR and focal adhesion pathways were also targeted by the virus. These interactions suggest that HEV probably utilizes important proteins in carbohydrate metabolism, energy generation and iron homoeostasis in the host cells during its establishment.",

keywords = "Gene Ontology, Virus-host interaction, Yeast two hybrid",

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doi = "10.1016/j.virusres.2015.12.007",

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AU - Lole, Kavita S.

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N2 - Hepatitis E virus ORF1 encoded non-structural polyprotein (nsP) consist of multiple domains, namely: Methyltransferase, Y-domain, Protease, X-domain, Helicase and RNA dependent RNA polymerase. We have attempted to identify human liver cell proteins that are interacting with HEV ORF1 encoded functional domains by using Y2H screening. A total of 155 protein-protein interactions between HEV-ORF1 and human proteins were identified. Comparative analysis of the HEV-ORF1-Human interaction network with reconstructed human interactome showed that the cellular proteins interacting with HEV-ORF1 are central and interconnected. Enrichment analysis of Gene Ontology and cellular pathways showed that the viral proteins preferentially interacted with the proteins of metabolism and energy generation along with host immune response and ubiquitin proteasomal pathways. The mTOR and focal adhesion pathways were also targeted by the virus. These interactions suggest that HEV probably utilizes important proteins in carbohydrate metabolism, energy generation and iron homoeostasis in the host cells during its establishment.

AB - Hepatitis E virus ORF1 encoded non-structural polyprotein (nsP) consist of multiple domains, namely: Methyltransferase, Y-domain, Protease, X-domain, Helicase and RNA dependent RNA polymerase. We have attempted to identify human liver cell proteins that are interacting with HEV ORF1 encoded functional domains by using Y2H screening. A total of 155 protein-protein interactions between HEV-ORF1 and human proteins were identified. Comparative analysis of the HEV-ORF1-Human interaction network with reconstructed human interactome showed that the cellular proteins interacting with HEV-ORF1 are central and interconnected. Enrichment analysis of Gene Ontology and cellular pathways showed that the viral proteins preferentially interacted with the proteins of metabolism and energy generation along with host immune response and ubiquitin proteasomal pathways. The mTOR and focal adhesion pathways were also targeted by the virus. These interactions suggest that HEV probably utilizes important proteins in carbohydrate metabolism, energy generation and iron homoeostasis in the host cells during its establishment.

KW - Gene Ontology

KW - Virus-host interaction

KW - Yeast two hybrid

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ER -

Hepatitis E virus ORF1 encoded non structural protein-host protein interaction network

Abstract

Bibliographical note

Funding

UN SDGs

Keywords

Access to Document

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