Hepatitis E virus ORF1 encoded non structural protein-host protein interaction network

Nishant Kumar Ojha, Kavita S. Lole

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


Hepatitis E virus ORF1 encoded non-structural polyprotein (nsP) consist of multiple domains, namely: Methyltransferase, Y-domain, Protease, X-domain, Helicase and RNA dependent RNA polymerase. We have attempted to identify human liver cell proteins that are interacting with HEV ORF1 encoded functional domains by using Y2H screening. A total of 155 protein-protein interactions between HEV-ORF1 and human proteins were identified. Comparative analysis of the HEV-ORF1-Human interaction network with reconstructed human interactome showed that the cellular proteins interacting with HEV-ORF1 are central and interconnected. Enrichment analysis of Gene Ontology and cellular pathways showed that the viral proteins preferentially interacted with the proteins of metabolism and energy generation along with host immune response and ubiquitin proteasomal pathways. The mTOR and focal adhesion pathways were also targeted by the virus. These interactions suggest that HEV probably utilizes important proteins in carbohydrate metabolism, energy generation and iron homoeostasis in the host cells during its establishment.

Original languageEnglish
Pages (from-to)195-204
Number of pages10
JournalVirus Research
StatePublished - 2 Feb 2016
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2015 Elsevier B.V.


  • Gene Ontology
  • Virus-host interaction
  • Yeast two hybrid


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