Hemin/G-quadruplexes as DNAzymes for the fluorescent detection of DNA, aptamer-thrombin complexes, and probing the activity of glucose oxidase

Eyal Golub; Ronit Freeman; Angelica Niazov; Itamar Willner

doi:10.1039/c1an15596b

Hemin/G-quadruplexes as DNAzymes for the fluorescent detection of DNA, aptamer-thrombin complexes, and probing the activity of glucose oxidase

Eyal Golub
, Ronit Freeman
, Angelica Niazov
, Itamar Willner

Hebrew University of Jerusalem

Research output: Contribution to journal › Article › peer-review

39 Scopus citations

Abstract

Hemin/G-quadruplex catalyzes the H₂O₂-mediated oxidation of Amplex Red to the fluorescent product resorufin. This process is implemented to develop hairpin nucleic acid structures for the detection of DNA, to probe the catalytic activity of glucose oxidase, to use the thrombin-aptamer complex as a catalytic readout structure, and to quantitatively analyze telomere chain composition.

Original language	English
Pages (from-to)	4397-4401
Number of pages	5
Journal	Analyst
Volume	136
Issue number	21
DOIs	https://doi.org/10.1039/c1an15596b
State	Published - 7 Nov 2011
Externally published	Yes

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10.1039/c1an15596b

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abstract = "Hemin/G-quadruplex catalyzes the H2O2-mediated oxidation of Amplex Red to the fluorescent product resorufin. This process is implemented to develop hairpin nucleic acid structures for the detection of DNA, to probe the catalytic activity of glucose oxidase, to use the thrombin-aptamer complex as a catalytic readout structure, and to quantitatively analyze telomere chain composition.",

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AU - Golub, Eyal

AU - Freeman, Ronit

AU - Niazov, Angelica

AU - Willner, Itamar

PY - 2011/11/7

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AB - Hemin/G-quadruplex catalyzes the H2O2-mediated oxidation of Amplex Red to the fluorescent product resorufin. This process is implemented to develop hairpin nucleic acid structures for the detection of DNA, to probe the catalytic activity of glucose oxidase, to use the thrombin-aptamer complex as a catalytic readout structure, and to quantitatively analyze telomere chain composition.

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Hemin/G-quadruplexes as DNAzymes for the fluorescent detection of DNA, aptamer-thrombin complexes, and probing the activity of glucose oxidase

Abstract

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