Guided protocol for fecal microbial characterization by 16S rRNA-amplicon sequencing

Keyphrases

• Fecal Microbial 100%
• 16S rRNA Gene Amplicon Sequencing 100%
• Microbial Characterization 100%
• Shotgun Sequencing 60%
• 16S rRNA Gene 40%
• Microbial Composition 40%
• QIIME 40%
• Analysis pipeline 20%
• High-throughput Sequencing 20%
• Functional Annotation 20%
• Sources of Variation 20%
• Human Disease 20%
• Dysbiosis 20%
• Pathologic Conditions 20%
• International Efforts 20%
• Human Health 20%
• Gut Microbiota Composition 20%
• Fecal Samples 20%
• Batch Effects 20%
• Targeted Sequencing 20%
• Sequence Method 20%
• DNA Extraction 20%
• Sequence Difference 20%
• Common Source 20%
• Vital Functions 20%
• Human Gut Microbiota 20%
• PCR Amplification 20%
• Healthy Microbiome 20%
• V3-V4 Region 20%
• Taxonomic Composition 20%
• Direct PCR 20%
• Gene Prediction 20%
• DADA2 20%
• Processing Energy 20%
• Microbial Fingerprint 20%
• Fecal Sample Collection 20%

Biochemistry, Genetics and Molecular Biology

• Amplicon Sequencing 100%
• 16S Ribosomal RNA 100%
• Shotgun Sequencing 42%
• Polymerase Chain Reaction 28%
• High Throughput Sequencing 14%
• Taxon 14%
• Microflora 14%
• Microbiome 14%
• DNA Extraction 14%