Graphene Oxide-Doped Gellan Gum–PEGDA Bilayered Hydrogel Mimicking the Mechanical and Lubrication Properties of Articular Cartilage

Diego Trucco, Lorenzo Vannozzi, Eti Teblum, Madina Telkhozhayeva, Gilbert Daniel Nessim, Saverio Affatato, Hind Al-Haddad, Gina Lisignoli, Leonardo Ricotti

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55 Scopus citations


Articular cartilage (AC) is a specialized connective tissue able to provide a low-friction gliding surface supporting shock-absorption, reducing stresses, and guaranteeing wear-resistance thanks to its structure and mechanical and lubrication properties. Being an avascular tissue, AC has a limited ability to heal defects. Nowadays, conventional strategies show several limitations, which results in ineffective restoration of chondral defects. Several tissue engineering approaches have been proposed to restore the AC's native properties without reproducing its mechanical and lubrication properties yet. This work reports the fabrication of a bilayered structure made of gellan gum (GG) and poly (ethylene glycol) diacrylate (PEGDA), able to mimic the mechanical and lubrication features of both AC superficial and deep zones. Through appropriate combinations of GG and PEGDA, cartilage Young's modulus is effectively mimicked for both zones. Graphene oxide is used as a dopant agent for the superficial hydrogel layer, demonstrating a lower friction than the nondoped counterpart. The bilayered hydrogel's antiwear properties are confirmed by using a knee simulator, following ISO 14243. Finally, in vitro tests with human chondrocytes confirm the absence of cytotoxicity effects. The results shown in this paper open the way to a multilayered synthetic injectable or surgically implantable filler for restoring AC defects.

Original languageEnglish
Article number2001434
JournalAdvanced healthcare materials
Issue number7
StatePublished - 7 Apr 2021

Bibliographical note

Publisher Copyright:
© 2021 The Authors. Advanced Healthcare Materials published by Wiley-VCH GmbH


D.T. and L.V. contributed equally to this work. This work received funding from the European Union's Horizon 2020 research and innovation program, grant agreement No 814413, project ADMAIORA (Advanced nanocomposite materials for in situ treatment and ultRAsound‐mediated management of osteoarthritis).

FundersFunder number
Horizon 2020 Framework Programme
Horizon 2020814413


    • cartilage lubrication properties
    • cartilage mechanical properties
    • cartilage substitutes
    • gellan gum
    • graphene oxide
    • hydrogels
    • polyethylene glycol diacrylate


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