TY - JOUR
T1 - Grapevine virus A-mediated gene silencing in Nicotiana benthamiana and Vitis vinifera
AU - Muruganantham, Mookkan
AU - Moskovitz, Yoni
AU - Haviv, Sabrina
AU - Horesh, Tamar
AU - Fenigstein, Annie
AU - Preez, Jacques du
AU - Stephan, Dirk
AU - Burger, Johan T.
AU - Mawassi, Munir
PY - 2009/2
Y1 - 2009/2
N2 - Virus-induced gene silencing (VIGS) is an attractive approach for studying gene function. Although the number of virus vectors available for use in VIGS experiments has increased in recent years, most of these vectors are applied in annual or herbaceous plants. The aim of this work was to develop a VIGS vector based on the Grapevine virus A (GVA), which is a member of the genus Vitivirus, family Flexiviridae. The GVA vector was used to silence the endogenous phytoene desaturase (PDS) gene in Nicotiana benthamiana plants. In addition, an Agrobacterium-mediated method for inoculating micropropagated Vitis vinifera cv. Prime plantlets via their roots was developed. Using this method, it was possible to silence the endogenous PDS gene in V. vinifera plantlets. The GVA-derived VIGS vector may constitute an important tool for improving functional genomics in V. vinifera.
AB - Virus-induced gene silencing (VIGS) is an attractive approach for studying gene function. Although the number of virus vectors available for use in VIGS experiments has increased in recent years, most of these vectors are applied in annual or herbaceous plants. The aim of this work was to develop a VIGS vector based on the Grapevine virus A (GVA), which is a member of the genus Vitivirus, family Flexiviridae. The GVA vector was used to silence the endogenous phytoene desaturase (PDS) gene in Nicotiana benthamiana plants. In addition, an Agrobacterium-mediated method for inoculating micropropagated Vitis vinifera cv. Prime plantlets via their roots was developed. Using this method, it was possible to silence the endogenous PDS gene in V. vinifera plantlets. The GVA-derived VIGS vector may constitute an important tool for improving functional genomics in V. vinifera.
KW - Agroinoculation
KW - Grapevine
KW - Grapevine virus A
KW - Phytoene desaturase
KW - Virus vector
KW - Virus-induced gene silencing
UR - http://www.scopus.com/inward/record.url?scp=57749198637&partnerID=8YFLogxK
U2 - 10.1016/j.jviromet.2008.10.010
DO - 10.1016/j.jviromet.2008.10.010
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C2 - 19010356
AN - SCOPUS:57749198637
SN - 0166-0934
VL - 155
SP - 167
EP - 174
JO - Journal of Virological Methods
JF - Journal of Virological Methods
IS - 2
ER -