Grapevine virus A-mediated gene silencing in Nicotiana benthamiana and Vitis vinifera

Mookkan Muruganantham, Yoni Moskovitz, Sabrina Haviv, Tamar Horesh, Annie Fenigstein, Jacques du Preez, Dirk Stephan, Johan T. Burger, Munir Mawassi

Research output: Contribution to journalArticlepeer-review

59 Scopus citations

Abstract

Virus-induced gene silencing (VIGS) is an attractive approach for studying gene function. Although the number of virus vectors available for use in VIGS experiments has increased in recent years, most of these vectors are applied in annual or herbaceous plants. The aim of this work was to develop a VIGS vector based on the Grapevine virus A (GVA), which is a member of the genus Vitivirus, family Flexiviridae. The GVA vector was used to silence the endogenous phytoene desaturase (PDS) gene in Nicotiana benthamiana plants. In addition, an Agrobacterium-mediated method for inoculating micropropagated Vitis vinifera cv. Prime plantlets via their roots was developed. Using this method, it was possible to silence the endogenous PDS gene in V. vinifera plantlets. The GVA-derived VIGS vector may constitute an important tool for improving functional genomics in V. vinifera.

Original languageEnglish
Pages (from-to)167-174
Number of pages8
JournalJournal of Virological Methods
Volume155
Issue number2
DOIs
StatePublished - Feb 2009
Externally publishedYes

Keywords

  • Agroinoculation
  • Grapevine
  • Grapevine virus A
  • Phytoene desaturase
  • Virus vector
  • Virus-induced gene silencing

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