Granulocyte colony-shmulatino factor (O-CSF) activation of stat3α and stat3β in normal and leuqmk human myeloid cells

G:SFulb.cytolilaiocJfordir6i,DJUiluktnoitocyUdiffmUlion. Acute my clod kubm (AML) clk. tick tnqMUly in from thii liMp, pond .brandy u OCSF by pnïfMi wUnt dilhnaitt. Th taiii far tt ibBoni npoaK unknown. Wg nooly aaooMttd Ibl bind, of O-CSF lo nonml jMd coD divud DNA bimlinj mplu, O-SIP-A, coined ofdienet SttU-ük. pnuu, SmO; m codmt, OCSF ctinled both SM3op92) god SMO I tana Iwbaic cU lin. AML-I93. A ibort fora of SM3 (SUUfl bu noWlr IMO dtKribld by ' lurfn t al. (/92:9097-9101,1995) Au heb th. J5 Cnid r~mi of o kv I b vn C4cml tuidut. jot pril i 3M3. W. .vMäpl H kypolhii H O-CSF iifuuni ii mml ad Iwbue bM myrfoid oik diver bwl of STAT fiaum aivKioB by .ummn, U coopo rf O-SIF-A in amd d taboic yrfoid odb. We donoubiud dal O-SIF-A ogAnid SOU m d AML ooU Ik Mot HL-60 pnvkudy duuuumd to diBbnaito k nqn to O-CSF mi n tkn of ii AML punt Mmpta. AcdvMionrf 9I3ocomlrfiiHnip(tm)oc.iiiDalotiofwliDl ccU mncU (WCE) md it iiMnuoopreciAl of du lyntot UM oilkibr espnuionofSUI3rilaiiwMiulliMlbriliiclivMioiL WCB of noand bnwi CD34 booc BBID cnlU d HL-60 odk era dncid of S4.ua; O-SIF-A wiken Da orik conBoudofonlyStatV. SbO mootinlid to bfed DNA in iMpo- to OOP. Tbm rowb Uiau IbM a SIM3-lik. praMio prariomly dniMid Su U eonnond, but m put, of SU13S od OM Ih O-SIF-A oonpo of dUÉtt SW3 ofanDi bun myrioid 1U VT~fa o ar Ai to ililTliiMiili h ~po O:SP. ActiY of Sut3o in ion AML Hi ny aUfna th ion of SW30 I kUcfW. wtt mmil myeloid dUfwwtutioo.