Gold Nanorods Based Air Scanning Electron Microscopy and Diffusion Reflection Imaging for Mapping Tumor Margins in Squamous Cell Carcinoma

Rinat Ankri, Ariel Ashkenazy, Yonat Milstein, Yaniv Brami, Asaf Olshinka, Nitza Goldenberg-Cohen, Aron Popovtzer, Dror Fixler, Abraham Hirshberg

Research output: Contribution to journalArticlepeer-review

51 Scopus citations

Abstract

A critical challenge arising during a surgical procedure for tumor removal is the determination of tumor margins. Gold nanorods (GNRs) conjugated to epidermal growth factor receptors (EGFR) (GNRs-EGFR) have long been used in the detection of cancerous cells as the expression of EGFR dramatically increases once the tissue becomes cancerous. Optical techniques for the identification of these GNRs-EGFR in tumor are intensively developed based on the unique scattering and absorption properties of the GNRs. In this study, we investigate the distribution of the GNRs in tissue sections presenting squamous cell carcinoma (SCC) to evaluate the SCC margins. Air scanning electron microscopy (airSEM), a novel, high resolution microscopy is used, enabling to localize and actually visualize nanoparticles on the tissue. The airSEM pictures presented a gradient of GNRs from the tumor to normal epithelium, spread in an area of 1 mm, suggesting tumor margins of 1 mm. Diffusion reflection (DR) measurements, performed in a resolution of 1 mm, of human oral SCC have shown a clear difference between the DR profiles of the healthy epithelium and the tumor itself.

Original languageEnglish
Pages (from-to)2349-2356
Number of pages8
JournalACS Nano
Volume10
Issue number2
DOIs
StatePublished - 23 Feb 2016

Bibliographical note

Publisher Copyright:
© 2016 American Chemical Society.

Keywords

  • EGFR bio conjugating
  • airSEM
  • diffusion reflection imaging
  • gold nanoparticles
  • oral cancer
  • squamous cells carcinoma
  • tumor margins

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