Gold nanoparticles for multimodal high-resolution imaging of transplanted cells for retinal replacement therapy

Yoav Chemla; Oshra Betzer; Amos Markus; Nairouz Farah; Menachem Motiei; Rachela Popovtzer; Yossi Mandel

doi:10.2217/nnm-2018-0299

Gold nanoparticles for multimodal high-resolution imaging of transplanted cells for retinal replacement therapy

Yoav Chemla, Oshra Betzer, Amos Markus, Nairouz Farah, Menachem Motiei, Rachela Popovtzer, Yossi Mandel

Research output: Contribution to journal › Article › peer-review

49 Scopus citations

Abstract

Aim: Longitudinal tracking of transplanted cells in clinical and experimental setups is crucial for evaluating the efficiency of retinal cell replacement therapies. Materials & methods: Gold nanoparticle-labeled photoreceptor precursors were transplanted in the vitreous and subretinal space of rats and were longitudinally tracked for over a month using optical coherence tomography, computed tomography and fluorescence fundus imaging. Results: This multimodal imaging approach enabled high-resolution long-term tracking and estimation of cell survival in the retina and vitreous, while displaying no toxic effects on the cells or the retina. Conclusion: These observations highlight the applicability of using gold nanoparticle for retinal cell tracking in existing experimental settings and its translational potential for providing more efficient retinal cell therapy in humans.

Original language	English
Pages (from-to)	1857-1871
Number of pages	15
Journal	Nanomedicine
Volume	14
Issue number	14
DOIs	https://doi.org/10.2217/nnm-2018-0299
State	Published - Jul 2019

Bibliographical note

Publisher Copyright:
© 2019 Future Medicine Ltd.

Funding

This work was supported by the Israeli Ministry of Science, Technology & Space doctoral and postdoctoral scholarships (Y Chemla and O Betzer), by the Israeli Council for Higher Education postdoctoral scholarship (O Betzer), by the Israeli Ministry of Defense (Y Mandel), by an ERC starter grant to Y Mandel and by the Israel Science Foundation ISF 749/14 (R Popovtzer). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. No writing assistance was utilized in the production of this manuscript.

Funders	Funder number
Israeli Council for Higher Education
Israeli Ministry of Defense
Israeli Ministry of Science
European Commission
Israel Science Foundation	ISF 749/14

Keywords

CT imaging
OCT imaging
cell tracking
cell transplantation
gold nanoparticles
retina

Access to Document

10.2217/nnm-2018-0299

Cite this

@article{f994e3f70f23429c96d5383cb2546e1a,

title = "Gold nanoparticles for multimodal high-resolution imaging of transplanted cells for retinal replacement therapy",

abstract = "Aim: Longitudinal tracking of transplanted cells in clinical and experimental setups is crucial for evaluating the efficiency of retinal cell replacement therapies. Materials & methods: Gold nanoparticle-labeled photoreceptor precursors were transplanted in the vitreous and subretinal space of rats and were longitudinally tracked for over a month using optical coherence tomography, computed tomography and fluorescence fundus imaging. Results: This multimodal imaging approach enabled high-resolution long-term tracking and estimation of cell survival in the retina and vitreous, while displaying no toxic effects on the cells or the retina. Conclusion: These observations highlight the applicability of using gold nanoparticle for retinal cell tracking in existing experimental settings and its translational potential for providing more efficient retinal cell therapy in humans.",

keywords = "CT imaging, OCT imaging, cell tracking, cell transplantation, gold nanoparticles, retina",

author = "Yoav Chemla and Oshra Betzer and Amos Markus and Nairouz Farah and Menachem Motiei and Rachela Popovtzer and Yossi Mandel",

note = "Publisher Copyright: {\textcopyright} 2019 Future Medicine Ltd.",

year = "2019",

month = jul,

doi = "10.2217/nnm-2018-0299",

language = "אנגלית",

volume = "14",

pages = "1857--1871",

journal = "Nanomedicine",

issn = "1743-5889",

publisher = "Taylor and Francis Ltd.",

number = "14",

}

TY - JOUR

T1 - Gold nanoparticles for multimodal high-resolution imaging of transplanted cells for retinal replacement therapy

AU - Chemla, Yoav

AU - Betzer, Oshra

AU - Markus, Amos

AU - Farah, Nairouz

AU - Motiei, Menachem

AU - Popovtzer, Rachela

AU - Mandel, Yossi

PY - 2019/7

Y1 - 2019/7

N2 - Aim: Longitudinal tracking of transplanted cells in clinical and experimental setups is crucial for evaluating the efficiency of retinal cell replacement therapies. Materials & methods: Gold nanoparticle-labeled photoreceptor precursors were transplanted in the vitreous and subretinal space of rats and were longitudinally tracked for over a month using optical coherence tomography, computed tomography and fluorescence fundus imaging. Results: This multimodal imaging approach enabled high-resolution long-term tracking and estimation of cell survival in the retina and vitreous, while displaying no toxic effects on the cells or the retina. Conclusion: These observations highlight the applicability of using gold nanoparticle for retinal cell tracking in existing experimental settings and its translational potential for providing more efficient retinal cell therapy in humans.

AB - Aim: Longitudinal tracking of transplanted cells in clinical and experimental setups is crucial for evaluating the efficiency of retinal cell replacement therapies. Materials & methods: Gold nanoparticle-labeled photoreceptor precursors were transplanted in the vitreous and subretinal space of rats and were longitudinally tracked for over a month using optical coherence tomography, computed tomography and fluorescence fundus imaging. Results: This multimodal imaging approach enabled high-resolution long-term tracking and estimation of cell survival in the retina and vitreous, while displaying no toxic effects on the cells or the retina. Conclusion: These observations highlight the applicability of using gold nanoparticle for retinal cell tracking in existing experimental settings and its translational potential for providing more efficient retinal cell therapy in humans.

KW - CT imaging

KW - OCT imaging

KW - cell tracking

KW - cell transplantation

KW - gold nanoparticles

KW - retina

UR - http://www.scopus.com/inward/record.url?scp=85069873895&partnerID=8YFLogxK

U2 - 10.2217/nnm-2018-0299

DO - 10.2217/nnm-2018-0299

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

C2 - 31339056

SN - 1743-5889

VL - 14

SP - 1857

EP - 1871

JO - Nanomedicine

JF - Nanomedicine

IS - 14

ER -

Gold nanoparticles for multimodal high-resolution imaging of transplanted cells for retinal replacement therapy

Abstract

Bibliographical note

Funding

Keywords

Access to Document

Other files and links

Fingerprint

Cite this