Gold nanoparticles: A novel application of spectral imaging u in proteomics - Preliminary results

Heidelinde R.C. Dietrich; Ian T. Young; Yuval Garini

doi:10.1117/12.587517

Gold nanoparticles: A novel application of spectral imaging u in proteomics - Preliminary results

Heidelinde R.C. Dietrich, Ian T. Young, Yuval Garini

Delft University of Technology

Research output: Contribution to journal › Conference article › peer-review

8 Scopus citations

Abstract

The intense research in proteomics is demanding for fast, reliable and easy-to-use methods in order to study the proteome. In this proceeding we report the development of such a novel research tool based on spectral imaging and Resonance Light Scattering gold particles. This method will allow the study of DNA-protein interactions. We suggest a broad range of applications: the screening of proteins binding to a specific DNA sequence, the analysis of binding affinities between protein and DNA, and the investigation of the influence of environmental conditions on the binding. We will explain the principle, first experiments and first results based on Brownian motion.

Original language	English
Article number	5694-14
Pages (from-to)	82-89
Number of pages	8
Journal	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	5694
DOIs	https://doi.org/10.1117/12.587517
State	Published - 2005
Externally published	Yes
Event	Spectral Imaging: Instrumentation Applications and Analysis III - San Jose, CA, United States Duration: 23 Jan 2005 → 23 Jan 2005

Keywords

DNA-Protein Interactions
Gold Nanoparticles
Protein-Protein Interactions
Proteomics
Single Molecule Detection
Surface Plasmons

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10.1117/12.587517

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abstract = "The intense research in proteomics is demanding for fast, reliable and easy-to-use methods in order to study the proteome. In this proceeding we report the development of such a novel research tool based on spectral imaging and Resonance Light Scattering gold particles. This method will allow the study of DNA-protein interactions. We suggest a broad range of applications: the screening of proteins binding to a specific DNA sequence, the analysis of binding affinities between protein and DNA, and the investigation of the influence of environmental conditions on the binding. We will explain the principle, first experiments and first results based on Brownian motion.",

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KW - Proteomics

KW - Single Molecule Detection

KW - Surface Plasmons

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Gold nanoparticles: A novel application of spectral imaging u in proteomics - Preliminary results

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Keywords

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