Genotyping DNA chip for the simultaneous assessment of antibiotic resistance and pathogenic potential of extraintestinal pathogenic Escherichia coli

Timo Barl; Ulrich Dobrindt; Xiaolei Yu; Don J. Katcoff; David Sompolinsky; Stéphane Bonacorsi; Jörg Hacker; Till T. Bachmann

doi:10.1016/j.ijantimicag.2008.04.020

Genotyping DNA chip for the simultaneous assessment of antibiotic resistance and pathogenic potential of extraintestinal pathogenic Escherichia coli

Timo Barl, Ulrich Dobrindt, Xiaolei Yu, Don J. Katcoff, David Sompolinsky, Stéphane Bonacorsi, Jörg Hacker, Till T. Bachmann

The Mina and Everard Goodman Faculty of Life Sciences

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

Urinary tract infections (UTIs) are among the most frequently occurring infections and are mostly caused by extraintestinal pathogenic Escherichia coli. DNA microarrays are potent molecular diagnostic tools for rapid diagnosis of bacterial infections with high relevance for UTIs. In this study, we present the integration and application of two DNA chip modules for the simultaneous detection of single nucleotide polymorphisms in gyrA (quinolone resistance) and fimH (increased adhesion to urinary tract epithelium). The performance of the combined diagnostic chip was assessed by genotyping 140 E. coli strains. Resistance-causing mutations could only be identified in UTI isolates. A complete genotyping assay could be performed in <4 h after DNA extraction. Together with the excellent genotyping results, this constitutes a competitive alternative as a standard tool for routine clinical diagnostics.

Original language	English
Pages (from-to)	272-277
Number of pages	6
Journal	International Journal of Antimicrobial Agents
Volume	32
Issue number	3
DOIs	https://doi.org/10.1016/j.ijantimicag.2008.04.020
State	Published - Sep 2008

Bibliographical note

Funding Information:
Funding: This work was funded within the PathoGenoMik research initiative by the German Federal Ministry of Education and Research (BMBF) and the ERA-NET project ‘Deciphering the intersection of commensal and extraintestinal pathogenic E. coli’.

Funding

Funding: This work was funded within the PathoGenoMik research initiative by the German Federal Ministry of Education and Research (BMBF) and the ERA-NET project ‘Deciphering the intersection of commensal and extraintestinal pathogenic E. coli’.

Funders	Funder number
Bundesministerium für Bildung und Forschung

Keywords

DNA microarray
Escherichia coli
Pathoadaptive mutation
Quinolone resistance
Urinary tract infection

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.ijantimicag.2008.04.020

Cite this

Barl, T., Dobrindt, U., Yu, X., Katcoff, D. J., Sompolinsky, D., Bonacorsi, S., Hacker, J., & Bachmann, T. T. (2008). Genotyping DNA chip for the simultaneous assessment of antibiotic resistance and pathogenic potential of extraintestinal pathogenic Escherichia coli. International Journal of Antimicrobial Agents, 32(3), 272-277. https://doi.org/10.1016/j.ijantimicag.2008.04.020

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abstract = "Urinary tract infections (UTIs) are among the most frequently occurring infections and are mostly caused by extraintestinal pathogenic Escherichia coli. DNA microarrays are potent molecular diagnostic tools for rapid diagnosis of bacterial infections with high relevance for UTIs. In this study, we present the integration and application of two DNA chip modules for the simultaneous detection of single nucleotide polymorphisms in gyrA (quinolone resistance) and fimH (increased adhesion to urinary tract epithelium). The performance of the combined diagnostic chip was assessed by genotyping 140 E. coli strains. Resistance-causing mutations could only be identified in UTI isolates. A complete genotyping assay could be performed in <4 h after DNA extraction. Together with the excellent genotyping results, this constitutes a competitive alternative as a standard tool for routine clinical diagnostics.",

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note = "Funding Information: Funding: This work was funded within the PathoGenoMik research initiative by the German Federal Ministry of Education and Research (BMBF) and the ERA-NET project {\textquoteleft}Deciphering the intersection of commensal and extraintestinal pathogenic E. coli{\textquoteright}. ",

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Barl, T, Dobrindt, U, Yu, X, Katcoff, DJ, Sompolinsky, D, Bonacorsi, S, Hacker, J & Bachmann, TT 2008, 'Genotyping DNA chip for the simultaneous assessment of antibiotic resistance and pathogenic potential of extraintestinal pathogenic Escherichia coli', International Journal of Antimicrobial Agents, vol. 32, no. 3, pp. 272-277. https://doi.org/10.1016/j.ijantimicag.2008.04.020

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AU - Bonacorsi, Stéphane

AU - Hacker, Jörg

AU - Bachmann, Till T.

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Genotyping DNA chip for the simultaneous assessment of antibiotic resistance and pathogenic potential of extraintestinal pathogenic Escherichia coli

Abstract

Bibliographical note

Funding

Keywords

UN SDGs

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