Genome-Wide identification of human RNA editing sites by parallel DNA capturing and sequencing

Jin Billy Li, Erez Y. Levanon, Jung Ki Yoon, John Aach, Bin Xie, Emily LeProust, Kun Zhang, Yuan Gao, George M. Church

Research output: Contribution to journalArticlepeer-review

435 Scopus citations

Abstract

Adenosine-to-inosine (A-to-I) RNA editing leads to transcriptome diversity and is important for normal brain function. To date, only a handful of functional sites have been identified in mammals. We developed an unbiased assay to screen more than 36,000 computationally predicted nonrepetitive A-to-I sites using massively parallel target capture and DNA sequencing. A comprehensive set of several hundred human RNA editing sites was detected by comparing genomic DNA with RNAs from seven tissues of a single individual. Specificity of our profiling was supported by observations of enrichment with known features of targets of adenosine deaminases acting on RNA (ADAR) and validation by means of capillary sequencing. This efficient approach greatly expands the repertoire of RNA editing targets and can be applied to studies involving RNA editing-related human diseases.

Original languageEnglish
Pages (from-to)1210-1213
Number of pages4
JournalScience
Volume324
Issue number5931
DOIs
StatePublished - 29 May 2009
Externally publishedYes

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