Genome of a giant bacteriophage from a decaying Trichodesmium bloom

Ulrike Pfreundt; Dina Spungin; Shengwei Hou; Björn Voß; Ilana Berman-Frank; Wolfgang R. Hess

doi:10.1016/j.margen.2017.02.001

Genome of a giant bacteriophage from a decaying Trichodesmium bloom

Ulrike Pfreundt, Dina Spungin, Shengwei Hou, Björn Voß, Ilana Berman-Frank, Wolfgang R. Hess

The Mina and Everard Goodman Faculty of Life Sciences

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

De-novo assembly of a metagenomic dataset obtained from a decaying cyanobacterial Trichodesmium bloom from the New Caledonian lagoon resulted in a complete giant phage genome of 257,908 bp, obtained independently with multiple assembly tools. Noteworthy, gammaproteobacteria were an abundant fraction in the sequenced samples. Mapping of the raw reads with 99% accuracy to the giant phage genome resulted in an average coverage of 262X. The closest sequenced relatives, albeit still distant, are the Pseudomonas phages PaBG from Lake Baikal and Lu11 isolated from a soil sample from the Philippines. The phage reported here might belong to the same family within the Myoviridae as PaBG and Lu11 and would thus be its first marine member, indicating a more widespread occurrence of this group. We named this phage NCTB (New Caledonia Trichodesmium Bloom) after its origin.

Original language	English
Pages (from-to)	21-25
Number of pages	5
Journal	Marine Genomics
Volume	33
DOIs	https://doi.org/10.1016/j.margen.2017.02.001
State	Published - Jun 2017

Bibliographical note

Publisher Copyright:
© 2017 Elsevier B.V.

Funding

This work was supported by the German-Israeli Research Foundation, project number 1133-13.8/2011 to WRH and IBF, by the EU project MaCuMBA (Marine Microorganisms: Cultivation Methods for Improving their Biotechnological Applications; grant agreement no: 311975) to WRH and by a China Scholarship Council grant no 201304910322 to SWH. We thank Tobias Wieland for preliminary data analysis and Damir Stazic for helpful Python scripts.

Funders	Funder number
European Commission	311975
German-Israeli Foundation for Scientific Research and Development	1133-13.8/2011
China Scholarship Council	201304910322

Keywords

Giant phage
Marine cyanobacteria
Metagenomics
Microbial community
Viral genome

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.margen.2017.02.001

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abstract = "De-novo assembly of a metagenomic dataset obtained from a decaying cyanobacterial Trichodesmium bloom from the New Caledonian lagoon resulted in a complete giant phage genome of 257,908 bp, obtained independently with multiple assembly tools. Noteworthy, gammaproteobacteria were an abundant fraction in the sequenced samples. Mapping of the raw reads with 99% accuracy to the giant phage genome resulted in an average coverage of 262X. The closest sequenced relatives, albeit still distant, are the Pseudomonas phages PaBG from Lake Baikal and Lu11 isolated from a soil sample from the Philippines. The phage reported here might belong to the same family within the Myoviridae as PaBG and Lu11 and would thus be its first marine member, indicating a more widespread occurrence of this group. We named this phage NCTB (New Caledonia Trichodesmium Bloom) after its origin.",

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AU - Spungin, Dina

AU - Hou, Shengwei

AU - Voß, Björn

AU - Berman-Frank, Ilana

AU - Hess, Wolfgang R.

PY - 2017/6

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KW - Giant phage

KW - Marine cyanobacteria

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KW - Microbial community

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Genome of a giant bacteriophage from a decaying Trichodesmium bloom

Abstract

Bibliographical note

Funding

Keywords

UN SDGs

Access to Document

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