TY - JOUR
T1 - Genetically engineered pfabA pfabR bacteria
T2 - An electrochemical whole cell biosensor for detection of water toxicity
AU - Neufeld, Tova
AU - Biran, Dvora
AU - Popovtzer, Rachela
AU - Erez, Tamir
AU - Ron, Eliora Z.
AU - Rishpon, Judith
PY - 2006/7/15
Y1 - 2006/7/15
N2 - We describe here a bacterial sensor for electrochemical detection of toxic chemicals. The sensor constitutes recombinant bacteria harboring plasmids encoding the fabA and fabR genes and has high-resolution amperometric response to membrane-damaging chemicals. For example, it can detect phenol at concentrations ranging between 1.6 and 16 ppm within 20 min. The high sensitivity is achieved by using the fabA promoter fused to a reporter gene-encoded β-galactosidase on a low copy number plasmid, under the control of the FabR repressor. The use of electrochemical whole cell sensors enables sensitive, fast, easy to operate, and cost-effective detection of water toxicity threats.
AB - We describe here a bacterial sensor for electrochemical detection of toxic chemicals. The sensor constitutes recombinant bacteria harboring plasmids encoding the fabA and fabR genes and has high-resolution amperometric response to membrane-damaging chemicals. For example, it can detect phenol at concentrations ranging between 1.6 and 16 ppm within 20 min. The high sensitivity is achieved by using the fabA promoter fused to a reporter gene-encoded β-galactosidase on a low copy number plasmid, under the control of the FabR repressor. The use of electrochemical whole cell sensors enables sensitive, fast, easy to operate, and cost-effective detection of water toxicity threats.
UR - http://www.scopus.com/inward/record.url?scp=33746260355&partnerID=8YFLogxK
U2 - 10.1021/ac052096r
DO - 10.1021/ac052096r
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C2 - 16841916
AN - SCOPUS:33746260355
SN - 0003-2700
VL - 78
SP - 4952
EP - 4956
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 14
ER -
