Generation of SMURF2 knockout human cells using the CRISPR/Cas9 system

Dhanoop Manikoth Ayyathan; Nataša Ilić; Hava Gil-Henn; Michael Blank

doi:10.1016/j.ab.2017.05.024

Generation of SMURF2 knockout human cells using the CRISPR/Cas9 system

Dhanoop Manikoth Ayyathan, Nataša Ilić, Hava Gil-Henn, Michael Blank

Bar-Ilan University - Azrieli Faculty of Medicine

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

The HECT domain E3 ubiquitin ligase SMURF2 regulates stability of several key protein targets involved in tumorigenesis, cell proliferation, migration, differentiation, and senescence. While altered levels and aberrant cellular distribution of SMURF2 were reported in different types of cancer, its role in tumorigenesis is far from understood. To elucidate the role of SMURF2 in cancer, appropriate human cancer cell models are needed. Here, we describe approaches that can be used to generate human normal and cancer cell strains knocked-out for SMURF2 using the clustered regularly interspaced short palindromic repeats (CRISPR/Cas9) gene-editing technology.

Original language	English
Pages (from-to)	56-59
Number of pages	4
Journal	Analytical Biochemistry
Volume	531
DOIs	https://doi.org/10.1016/j.ab.2017.05.024
State	Published - 15 Aug 2017

Bibliographical note

Publisher Copyright:
© 2017 Elsevier Inc.

Funding

This work was supported by the ICRF (grant number 00636), Marie-Curie FP-7 CIG (grant number 612816), the Israel Cancer Association (grant numbers 20160073 and 20171153), and Dayan Family Foundation (to M.B); and by the Israel Science Foundation (grant number 996/12), the Israel Cancer Association (grant number 20141043) and ICRF (grant number 12-709-RCDA) (to H.G.H.).

Funders	Funder number
Dayan Family Foundation
Marie-Curie FP-7 CIG	612816
Israel Cancer Research Fund	00636
Israel Cancer Association	20171153, 20160073
Israel Science Foundation	12-709-RCDA, 20141043, 996/12

Keywords

CRISPR/Cas9
Human cells
Knockout
SMURF2

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.ab.2017.05.024

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title = "Generation of SMURF2 knockout human cells using the CRISPR/Cas9 system",

abstract = "The HECT domain E3 ubiquitin ligase SMURF2 regulates stability of several key protein targets involved in tumorigenesis, cell proliferation, migration, differentiation, and senescence. While altered levels and aberrant cellular distribution of SMURF2 were reported in different types of cancer, its role in tumorigenesis is far from understood. To elucidate the role of SMURF2 in cancer, appropriate human cancer cell models are needed. Here, we describe approaches that can be used to generate human normal and cancer cell strains knocked-out for SMURF2 using the clustered regularly interspaced short palindromic repeats (CRISPR/Cas9) gene-editing technology.",

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doi = "10.1016/j.ab.2017.05.024",

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AU - Gil-Henn, Hava

AU - Blank, Michael

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AB - The HECT domain E3 ubiquitin ligase SMURF2 regulates stability of several key protein targets involved in tumorigenesis, cell proliferation, migration, differentiation, and senescence. While altered levels and aberrant cellular distribution of SMURF2 were reported in different types of cancer, its role in tumorigenesis is far from understood. To elucidate the role of SMURF2 in cancer, appropriate human cancer cell models are needed. Here, we describe approaches that can be used to generate human normal and cancer cell strains knocked-out for SMURF2 using the clustered regularly interspaced short palindromic repeats (CRISPR/Cas9) gene-editing technology.

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Generation of SMURF2 knockout human cells using the CRISPR/Cas9 system

Abstract

Bibliographical note

Funding

Keywords

UN SDGs

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