Generation of SMURF2 knockout human cells using the CRISPR/Cas9 system

Dhanoop Manikoth Ayyathan, Nataša Ilić, Hava Gil-Henn, Michael Blank

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

The HECT domain E3 ubiquitin ligase SMURF2 regulates stability of several key protein targets involved in tumorigenesis, cell proliferation, migration, differentiation, and senescence. While altered levels and aberrant cellular distribution of SMURF2 were reported in different types of cancer, its role in tumorigenesis is far from understood. To elucidate the role of SMURF2 in cancer, appropriate human cancer cell models are needed. Here, we describe approaches that can be used to generate human normal and cancer cell strains knocked-out for SMURF2 using the clustered regularly interspaced short palindromic repeats (CRISPR/Cas9) gene-editing technology.

Original languageEnglish
Pages (from-to)56-59
Number of pages4
JournalAnalytical Biochemistry
Volume531
DOIs
StatePublished - 15 Aug 2017

Bibliographical note

Publisher Copyright:
© 2017 Elsevier Inc.

Keywords

  • CRISPR/Cas9
  • Human cells
  • Knockout
  • SMURF2

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